非小细胞肺癌患者血清中MALAT1表达水平及其临床意义

Expression of MALAT1 in serum of the patients with non-small cell lung cancer(NSCLC) and its clinical significance

目的探讨肺腺癌转移相关转录因子1(MALAT1)在非小细胞肺癌(NSCLC)患者血清中的表达水平及其诊断价值。方法提取60例NSCLC患者、42例肺良性病变患者及50例健康人血清中的总RNA,用Taq Man实时荧光定量RT-PCR检测MALAT1基因的表达水平并分析其与NSCLC患者临床病理因素的关系,以受试者工作特征曲线(ROC)分析血清MALAT1的诊断效能。结果肺癌组血清MALAT1相对表达水平(0.51±0.09)低于肺良性病变组(0.80±0.12)及健康人对照组(1.01±0.30),差异有统计学意义(q分别为13.95、12.27,P<0.01);肺良性病变组血清MALAT1表达水平低于健康人对照组(q=10.24,P<0.01);腺癌患者血清MALAT1相对表达量(0.57±0.04)较鳞癌患者(0.45±0.07)明显增高(t=4.260,P<0.01),而与TNM分期、淋巴结转移和肿瘤分布部位无显著相关性(P>0.05);ROC曲线下面积(AUCROC)为0.956,当cut off值为0.62时,敏感性和特异性分别是87.5%和94.1%,诊断指数为181.6%。结论血清MALAT1检测效能好,可用于NSCLC的诊断及病理类型的鉴别。

Objective To investigate the expression level of metastasis associated lung adenocarcinoma transcript 1（ MALAT1） in serum of the patients with non-small cell lung cancer（ NSCLC） and its diagnostic value. Methods The total RNAs were extracted from serum samples of 60 patients with NSCLC,42 patients with benign lung lesions and 50 healthy controls,respectively. Then,the expression level of serum MALAT1 was detected by real-time PCR,and its correlation with clinical pathological parameters was analyzed.The diagnostic efficacy of serum MALAT1 was analyzed by the receiver operating characteristic（ ROC） curve. Results The relative expression level of MALAT1 in the patients with NSCLC（ 0. 51 ± 0. 09） was significantly lower than those in the patients with benign lung lesions（ 0. 80 ± 0. 12,q = 13. 95,P〈0. 01） and healthy controls（ 1. 01 ± 0. 30,q = 12. 27,P〈0. 01）. The expression level of MALAT1 in the patients with benign lung lesions was significantly lower than that in healthy controls（ q = 10. 24,P〈0. 01）. The relative expression level of serum MALAT1 in adenocarcinoma patients（ 0. 57 ± 0. 04） was significantly higher than that in squamous carcinoma patients（ 0. 45 ± 0. 07,t = 4. 260,P〈0. 01）. However,the expression level of serum MALAT1 was not correlated with TNM stage,lymphatic metastasis and tumor location（ P〉0. 05）. The area under the curve（ AUC^ROC） was 0. 956. When the cutoff value of serum MALAT1 level was set as 0. 62,the sensitivity and specificity were 87. 5% and 94. 1%,respectively,and the diagnostic index was 181. 6% for the diagnosis of NSCLC. Conclusion The detection of serum MALAT1 level may be helpful for the diagnosis of NSCLC and the differentiation of pathologic types of NSCLC.