锁阳多糖对肺癌细胞端粒的影响

Effect of Cynomorium songaricum polysaccharide on telomere of lung cancer A549 cells

该文主要研究锁阳多糖（Cynomorium songaricum polysaccharide,CSRP）对人非小细胞肺癌A549细胞端粒的影响。小鼠灌胃CSRP（0.08 g·kg-1）,1次/d,连续给药4 d后,取血清作为CSRP含药血清备用。含药血清处理A549细胞,采用MTT法测定CSRP在不同浓度和处理后不同时间对细胞增殖的变化。各浓度于含药血清处理后48 h荧光定量PCR法测定细胞端粒长度,RT-q PCR法测定端粒酶逆转录酶（telomerase reverse transcriptase,TERT）mRNA的表达,TUNEL法检测细胞凋亡的情况。研究发现,各浓度CRSP均可以显著抑制肺癌A549细胞的增殖,在48 h,6.0 m L·L-1时抑制作用达到最强;48 h时,1.5~12.0 m L·L-1各浓度CRSP均可显著缩短A549细胞端粒长度,1.5 m L·L-1浓度时缩短作用最明显;各浓度CRSP均可显著抑制A549细胞TERT mRNA的表达,浓度达到或超过6.0 m L·L-1时抑制作用更强;各浓度CSRP均可促进A549细胞的凋亡,在达到或超过6.0 m L·L-1时促进细胞凋亡作用更强。因此CSRP具有抗肿瘤作用,作用机制可能是通过抑制TERT mRNA表达,缩短端粒长度,抑制癌细胞增殖及促进癌细胞凋亡等机制实现。

To study the effect of Cynomorium songaricum polysaccharide（ CSRP） on A549 cells telomere of human non-small cell lung cancer,the mice were intragastric administrated with CSRP（ 0. 08 g·kg-1） once daily for 4 days. Then their serum was taken for preparing CSRP drug serum. A549 cells were treated by the drug serum,and the effect of drug serum with different concentrations and different treating time on the proliferation of non-small cell lung cancer A549 cells was determined by MTT test. After treating for 48 hours by the drug serum of different concentrations,the telomere length of the cells was determined by fluorescence quantitative polymerase chain reaction（ q PCR）; the mRNA expression of telomerase reverse transcriptase（ TERT） was determined by RT-q PCR; the cells apoptosis was determined by TUNEL assay. The results demonstrated that CSRP of various concentrations could inhibit the proliferation of the lung cancer A549 cells significantly,and the inhibition effect was strongest at 48 hours with the concentration of 6. 0 m L·L-1. At 48 h,that CSRP of the concentrations from 1. 5 to 12. 0 m L·L-1could significantly shorten the telomere length of A549 cells,and the effect was strongest with the concentration of 1. 5 mg·L-1. CSRP of various concentrations could significantly inhibit the mRNA expression of TERT in A549 cells,and the inhibition effect was stronger when the concentration was ≥6. 0 m L·L-1. CSRP of various concentrations could promote A549 cells apoptosis,and the effect was stronger when the concentration was ≥6. 0 m L·L-1. In conclusion,CSRP has the anti-cancer effect,and the action mechanism may be associated with inhibiting TERT mRNA expression,shortening telomere length,inhibiting cells proliferation and promoting cells apoptosis.