一个辣椒PHD-finger家族转录因子CaPHD1的克隆及其表达分析

Cloning and Expressional Characterization of Pepper CaPHD1 Gene

以辣椒均一化cDNA文库为模板,通过PCR扩增获得CaPHD1基因克隆,该基因序列含有1个220个氨基酸序列的开放阅读框,同其他植物具有62%-89%的氨基酸同源性。在植物亚细胞定位实验中CaPHD1与GFP的融合蛋白定位于细胞核;实时荧光定量PCR分析结果表明,该基因受到青枯菌侵染和水杨酸、茉莉酸甲酯、乙烯和脱落酸等外源激素的诱导,表明CaPHD1可能在应答病原菌中起重要的作用。

The plant homeodomain（PHD） zinc finger proteins are widely distributed in eukaryotes, and involved in plant growth, developments and anti-stress process. In the present study, a full length cDNA, designated as CaPHD1,was amplified by PCR with specific primers using normalized c DNA library of pepper as the template. An open reading frame encoding 220 amino acids was found the in the full length cDNA and its deduced amino acid sequence shared 62%-89% identity from other plant species. Subcellular localization assay of CaPHD1 by transient expressing 35S：：CaPHD1：：GFP in N. benthamiana leaves showed that exclusive localization of CaPHD1 protein in nuclei. Results of qRT-PCR analysis in pepper leaves against the inoculation of Ralstonia solanacearum and exogenous application of salicylic acid（ SA）, jasmonic acid（ Me JA）, abscisic acid（ ABA） and ethephon（ ETH）showed that CaPHD1 was transcriptionally upregulated to different degree by exogenous phytohormones applications of salicylic acid（ SA）, methyl jasmonic acid（ Me JA）, abscisic acid（ ABA） and ethephon（ ETH）, as well as by inoculation of R. solanacearum. These results suggested that CaPHD1 may play an important role in response to pathogen infection.