噁二唑罗丹明荧光染料对Hela细胞和U937细胞染色效果研究

The Staining Efficiency Study of Rhodamine-oxadiazole Fluorescent Dye to Hela Cells and U937 Cells

以合成的含噁二唑杂环的罗丹明荧光染料(Rh-M)对Hela和U937细胞进行了活体染色实验.荧光显微镜下观察该荧光染料可以在5 min内进入Hela细胞,染色后的细胞发光面积较大,强度较高,Rh-M浓度在10-20μmol/L,染色15 min效果最佳.激光扫描共聚焦显微镜(Confocal laser scanning microscope,CLSM)下,浓度在20μmol/L染色15 min的Hela细胞,可以清晰看出染料在细胞质中均匀分布.流式细胞术((flow cytometry,FCM)检测U937细胞,表明Rh-M与肿瘤细胞的亲和能力强,在100μmol/L内荧光亮度与荧光染料浓度呈现增加趋势.

In this research we proceeded a intravital staining process with the fluorescent rhodamine dye containing oxadiazoleheterocycles （Rh-M） on Hela cells and U937 cells. We observed that the Rh-M could enter the Hela cells within 5 min and the fluorescence signals after staining were widely spread and bright under the fluorescence microscope. The most effective staining condition when the stain concentration is between 10 and 20 μmol/L and the stain time is 15 min. Under the confocal laser scanning microscopy, the Hela cells which were stained with the 20 μmol/L Rh-M and stain 15 rain showed that Rh-M distributed uniformly in the cytoplasm. We also used flow cytometry to detect U937 cells, the results showed that the Rh-M had strong affinity to tumor cells. The staining results were satisfactory and within 100 μM the fluorescence intensity increased as the concentration of Rh-M increased.