KLF4对口腔鳞癌细胞增殖的抑制作用

The proliferation inhibitory effect of KLF4 in oral squamous cell carcinoma cells

目的探讨KLF4对人口腔鳞癌细胞CAL27的增殖抑制作用。方法构建KLF4慢病毒表达载体,并转染人口腔鳞癌细胞系CAL27,转染不含KLF4开放读码框的慢病毒载体LV105作为对照。采用RT-PCR、免疫细胞化学法对KLF4表达进行鉴定。MTT实验、流式细胞术检测KLF4对口腔鳞癌细胞系CAL27的增殖抑制作用。结果 RT-PCR、免疫细胞化学实验均表明实验组细胞KLF4的表达明显高于对照组(P<0.01)。MTT实验结果表明与对照组CAL27/LV105组相比,CAL27/KLF4能够抑制细胞的增殖能力(P<0.01)。CAL27/KLF4主要通过使细胞周期中的G2/M期阻滞来抑制CAL27细胞的增殖(P<0.01)。结论 KLF4转染能够抑制口腔鳞癌细胞系CAL27的增殖能力,可能在口腔鳞状细胞癌中作为抑癌基因发挥作用。

Objective To investigate the role of KLF4 in proliferation of oral squamous cell carcinoma（ OSCC）cell line CAL27. Methods OSCC cell line CAL27 was cultured in DMEM-High glucose medium with 10% FBS.Lentiviruses carrying KLF4 gene were constructed and transducted into CAL27 cells. Puromycin was used to select the stable cell line expressing KLF4 gene. RT-PCR and immunocytochemistry were used to identify the expression of KLF4.MTT and flow cytometry were performed to investigate the role of KLF4 in proliferation in stable transfection cells CAL27.Results The expression of KLF4 in CAL27 / KLF4 cells was significantly up regulated compared with CAL27 / LV105 by RT-PCR and immunocytochemistry（ P 〈0. 01）. Overexpression of KLF4 could inhibit the proliferation of CAL27 cells（ P 〈0. 01）. Moreover,The flow cytometry of cell cycle indicated that overexpression of KLF4 inhibited the proliferation of CAL27 mainly by arresting the G2 / M phase（ P 〈0. 01）. Conclusion CAL27 cells transduction with KLF4 could inhibit the proliferation of CAL27 cells in vitro,which may play a role as a suppressor gene in oral carcinogenesis.