特发性矮小症患儿血清生长素、瘦素、胰岛素样生长因子、胰岛素样生长因子结合蛋白表达水平及意义

The expression and significance of serum ghreHn, leptin, IGF-1, and IGFBP3 in children with idiopathic short stature

目的探讨特发性矮小症（idiopathic short stature，ISS）患儿血清生长素（Ghrelin）、瘦素（1eptin，LP）、胰岛素样生长因子-1（insulin-like growth factors-1，IGF-1）及胰岛素样生长因子结合蛋白-3（insulin—like growth factor bindingprotein-3，IGFBP3）表达水平及其在特发性矮小症患儿生长发育中的意义。方法选取2012年5月至2014年10月来富阳市妇幼保健院儿科治疗的40例特发性矮小症患者为研究对象．另选取40例同时期体检健康儿童为对照组，采用ELISA法测定血清Ghrelin、LP水平，采用免疫化学发光法检测血清IGF-1、IGFBP3水平，对测定结果进行统计分析。结果观察组ISS患儿身高、体重、BMI、GH、血清LP、IGF-1、IGFBP3表达水平显著低于对照组，血清Ghrelin表达水平显著高于对照组，差异具有统计学意义（P〈0．05）；特发性矮小症患儿血清Ghrelin与LP呈负相关（r=-0．611，P〈0．01），Ghrelin与IGF-1水平呈负相关（r=-0．520，P〈0．05），Ghrelin与IGFBP3呈正相关（r=0．586，P〈0．01），IGF-1与IGFBP3呈负相关（r=-0．576，P〈0．01），LP与IGFBP3呈负相关（r=-0．609，P〈0．01）。结论特发性矮小症患儿生长激素分泌状态与Ghrelin、LP、IGF-1、IGFBP3水平相关，Ghrelin与胰岛素样生长因子轴之间相互作用，调控生长发育。

Objective To examine the expressions of serum growth hormone （ghrelin）, leptin （LP）, in sulin-like growth factor-1 （IGF-1） and insulin-like growth factor binding protein-3 （IGFBP3） in children with idiopathic short stature （ISS）, and their significance. Methods A total of 40 patients with ISS were selected from May. 2012 to Oct. 2014 in Fuyang Maternal and Child Health Hospital, while a total of 40 children in good health were selected as the control group. Serum ghrelin and LP levels were measured by ELISA. Serum IGF-1 and IGF- BP3 levels were detected by chemiluminescence immune assay. Results were analyzed statistically. Results The height, weight, BMI, GH, serum LP, IGF-1, and IGFBP3 levels in the observation group were significantly lower than those in the control group, while serum ghrelin expression level was significantly higher than that in the control group（P〈0.05）. The correlation analysis showed that ghrelin and LP were negatively correlated（r=-0.611, P〈0.01 ）, ghrelin and IGF-1 levels were negatively correlated（r=-0.520, P〈0.05）, Ghrelin and IGFBP3 were pos- itively correlated （r=0.586, P〈0.01）, IGF-1 and IGFBP3 was negatively correlated （r=-0.576, P〈0.01）, and LP and IGFBP3 were negatively correlated （r=-0.609, P〈0.01 ）. Conclusions It shows that ghrelin, LP, IGF-1, and IGFBP3 levels in children with ISS are related to growth hormone secretion status. The interactions between ghre- lin and insulin-like growth factor axis regulate growth and development of children.