HPLC法同时测定尖叶假龙胆中三种山酮类化合物的含量

Quantitative Analysis of Three Xanthones in Gentianella Acuta by HPLC

目的：利用高效液相色谱法同时测定尖叶假龙胆中当药醇苷、雏菊叶龙胆酮、去甲基雏菊叶龙胆酮的含量。方法：取尖叶假龙胆粗粉0.5 g,用甲醇25 m L超声震荡提取30 min,制成供试品溶液。色谱条件：Waters Symmetry C18色谱柱（4.6 mm×150 mm,5μm）;流动相：甲醇-磷酸水（p H2.6）;柱温35℃;流速1 m L/min;进样量：10μL;梯度洗脱45 min。结果：尖叶假龙胆中当药醇苷、去甲基雏菊叶龙胆酮、雏菊叶龙胆酮分别在13.6~122.4μg/m L,10~90μg/m L,10.8~97.2μg/m L的范围内线性关系良好（r值分别为0.997 4,0.996 7,0.998 0）;当药醇苷、去甲基雏菊叶龙胆酮、雏菊叶龙胆酮平均回收率（n=6）分别为99.97%,102.02%,98%。结论：尖叶假龙胆中当药醇苷,去甲基雏菊叶龙胆酮和雏菊叶龙胆酮的含量分别为23.94 mg/g,4.49 mg/g,7.92 mg/g。

Objective： To simultaneously determinate the Swertianolin,bellifolin,desmethybellidifolin of Gentianella acuta by HPLC. Methods： The analysis was aehieved on an Waters Symmetry C18column（ 4. 6 mm × 250 mm,5 μm） by using a mobile phase of Me OH-Water（ p H2. 6） gradient elution in a total run time of 45 minutes and the detection wavelength was set at 252 nm. Results： Swertianolin,desmethybellidifolin and bellifolin were linear over the range of 13. 6 ~122. 4 μg / ml,10 ~ 90 μg / ml,10. 8 ~ 97. 2 μg / ml（ r was 0. 997 4,0. 996 7,0. 998 0）. The average recoveries（ n = 6）were 99. 97%,102. 02% and 98%. Conclusion： The contents of swertianolin,desmethybellidifolin and bellifolin in Gentianella acuta were 23. 94 mg / g,4. 49 mg / g and 7. 92 mg / g.