维医异常黏液质型阳痿病证大鼠模型阴茎组织中ET-1的改变及其生物学意义

Biological significance and variation of endothelin-1 in the penis of rat model with abnormal phlegmatic syndrome

目的研究维医异常黏液质型阳痿病证大鼠模型阴茎组织中ET-1的改变及其生物学意义。方法 100只SD大鼠经实验确认具有正常性功能后,10只作为正常对照组,其余90只作为造模组,采用湿寒性饲料和湿寒性环境条件下建立异常黏液质证候模型。造模20w以后,通过交配和勃起实验从证候模型中筛选出阳痿病证模型,并将其分为病证模型组和病证药物反证组,未成阳痿的证候模型再随机分为证候模型组与证候药物反证组。药物干预2周后,取材,并对阴茎组织进行免疫组化染色,光镜下观察。结果证候模型组、病证模型组大鼠阴茎组织中ET-1的表达均较正常对照组显著增高(P<0.05),各组织间差异均无统计学意义(P>0.05),而证候药物反证组和病证药物反证组ET-1较证候模型组和病证模型组明显降低,但无统计学差异(P>0.05)。结论 ET-1表达水平升高可能是异常黏液质型阳痿病证重要的发病机制之一。

Objective To study the biological significance and variation of endothelin-1expression in the penis of rat model with impotence disease.Methods A total of 100 normal mature male SD rats were enrolled in the study,and 10 of them were randomly chosen as the control group,the other 90 were treated with spinach and coriander diet in a cold and humid environment until the model of abnormal phlegmatic syndrome was established.After 20 weeks of diet treatment,they were divided into abnormal phlegmatic syndrome model group and pharmacological intervention group by APO erectile experiments and mating experiments.After 2weeks of recovery treatment to the pharmacological intervention group,immunohistochemical stainingwas conducted in the penile tissue of all groups.Results Compared withthe control group,the level of endothelin-1expression in the impotence model group,medication group of impotence model,abnormal balgam syndrome group was increased significantly（P〈0.05）.Each of group have no obvious difference（P〉0.05）.After medication intervention,the level of endothelin-1in medication group of abnormal balgam syndrome and medication group of impotence model were both decreased significantly.Conclusion The level of endothelin-1expression on abnormal mucinous impotence disease syndrome of rats increased significantly.