人乳头瘤病毒感染女性配偶精液参数及抗精子抗体检测分析

Analysis of semen parameters and anti-sperm antibodies in male whose spouses infected with human papilloma virus

目的探讨人乳头瘤病毒（HPV）感染女性的配偶精液HPV感染状况以及其对精子参数和抗精子抗体（AsAb）的影响。方法收集2014年1月—2015年12月在华北石油管理局总医院进行精液检测的125例已婚男性为研究对象,其配偶均经宫颈组织检测确定为HPV感染。分别对男性的精液标本进行常规分析及HPV、AsAb检测。结果 HPV感染女性的配偶精液HPV感染阳性率为42.4%（53/125）,其中单一型感染49例（92.5%）,二重感染3例（5.7%）,多重感染1例（1.8%）,夫妻HPV吻合率为15.2%（19/125）。HPV感染男性的精子活力低于非感染男性（t=2.928,P=0.004）,而精子密度、精子存活率、精子畸形率差异无统计学意义（P〉0.05）。HPV感染男性精液AsAb阳性率高于非感染男性（34.0%vs.18.1%,χ^2=4.142,P=0.042）。结论 HPV感染女性的配偶也是病毒感染的高危人群,特别是对于有生育愿望的夫妇,应关注感染对男性生育的影响。

Objective To explore the infection status in male whose wife infected with human papilloma virus and the impact on sperm parameters and anti-sperm antibodies（AsAb）.Methods From December 2015 to January 2014,125 cases of married men in the General Hospital of North China Petroleum Administration were collected as study subjects.Their spouses were identified as HPV infection by the detection of cervical tissue.Routine analysis of semen samples and HPV,AsAb detection were performed.Results The HPV infection of female spouses semen HPV infection positive rate was 42.4%（53/125）,the single type infection in 49 cases（92.5%）,dual infection in 3 patients（5.7%）,multiple infection in 1 case（1.8%）,the husband and wife HPV agreement rate was 15.2%（19/125）.Sperm motility of male HPV infection was lower than that of non-infected males（t =2.928,P =0.004）,and sperm density,sperm survival rate,sperm malformation rate difference was not statistically significant（P〉0.05）,HPV infection in semen AsAb positive rate higher than in non-infected males（34.0%vs.18.1%,χ^2 =4.142,P =0.042）.Conclusion Male whose wife infected with HPV is at high risk of infection.It is necessary to pay more attention to the influence of infection on male fertility for couples who have fertility willingness.