摘要
[Objective] This study was conducted to clone zmERECTA gene according to Arabidopsis ERECTA sequence and predict its characteristics by bioinformatics. [Method] The c DNA of zmERECTA gene was isolated from B73 using RT-PCR, and analyzed by bioinformatics methods. [Result] zmERECTA gene was 2 985 bp in size, which encoded a protein consisting of 944 amino acids, containing leucine-rich repeats, a PKC domain, two transmembrane regions, 14 N-glycosylation potential sites and41 kinase specific phosphorylation sites. The theoretical p I and molecular weight of zmERECTA protein was 6.01 and 10 8495.5respectively. [Conclusion] Cloning and bioinformatics of zmERECTA gene laid a foundation for further research.
[Objective] This study was conducted to clone zmERECTA gene according to Arabidopsis ERECTA sequence and predict its characteristics by bioinformatics. [Method] The c DNA of zmERECTA gene was isolated from B73 using RT-PCR, and analyzed by bioinformatics methods. [Result] zmERECTA gene was 2 985 bp in size, which encoded a protein consisting of 944 amino acids, containing leucine-rich repeats, a PKC domain, two transmembrane regions, 14 N-glycosylation potential sites and41 kinase specific phosphorylation sites. The theoretical p I and molecular weight of zmERECTA protein was 6.01 and 10 8495.5respectively. [Conclusion] Cloning and bioinformatics of zmERECTA gene laid a foundation for further research.
基金
Supported by the Distinguished Young Scientists Project of Beijing(CIT&TCD201304096)
Academic Degrees and Graduate Education Reform and Development Program of Beijing University of Agriculture(5056516002\016)
