儿茶酚抑素对心梗大鼠心肌纤维化的影响

Effect of catechol endostatin on myocardial fibrosis in rats with myocardial infarction

目的探讨儿茶酚抑素(CST)治疗对心梗(MI)大鼠心肌纤维化的影响。方法结扎SD大鼠冠状动脉前降支建立心肌梗死模型,将模型大鼠随机分为儿茶酚抑素组、心梗对照组,每组16只,另设假手术组16只。术后1 d开始,儿茶酚抑素组连续3周腹腔注射儿茶酚抑素2 nmol/(kg·d),心梗对照组和假手术组给予等量生理盐水腹腔注射。4周后,记录左室血流动力学改变;处死大鼠,留取心脏标本,Masson染色检测心肌纤维化;Western blot法检测梗死周边Ⅰ胶原蛋白表达。结果与心梗对照组比较,儿茶酚抑素组的心功能损害较轻(P<0.01),梗死百分比及胶原含量显著降低(P<0.05,P<0.01)。结论儿茶酚抑素能改善心脏功能,并能减轻心梗后非梗死区反应性胶原的过多沉积,预防心梗后心肌纤维化。

Objective To investigate the effect of catechol endostatin on myocardial fibrosis in rats with myocardial infarction（MI）.Methods MI model of Sprague-Dawley（SD）rat was established by ligating the left anterior descending coronary artery.Rats were randomly divided into MI group（n = 16）and CST group（n = 16）,and 16 rats were chosen as sham group.Rats in CST group were given CST 2 nmol/（kg·d）ip for 3 weeks at first day after operation,while an equal amount of 0.9% normal saline was applied to MI group and sham group.The left ventricular hemodynamics was measured.Myocardial interstitial fibrosis was observed by Masson staining,and the peripheral collagenⅠ was detected by western blot.Results Compared with MI group,cardiac function damage was milder in CST group（P〈0.01）.Collagen content and infarct size decreased significantly（P〈0.05,P〈0.01）.Conclusion CST can improve the cardiac function and reduce reactive myocardial fibrosis,thus preventing myocardial fibrosis.