基于黄酮类成分对射干指纹图谱研究

Research of the Fingerprint of Rhizoma Belamcandae Based on the Flavonoids Ingredients

目的:研究不同产地射干的指纹图谱,建立共有模式图谱,为射干药材质量控制提供依据。方法:采用HPLC法测定,以乙腈-水作为流动相进行梯度洗脱,检测波长为265 nm。结果:建立了共有模式图谱,11份射干样品共有25个共有色谱峰,保留时间RSD≤0.77%,非共有峰保留时间RSD<2.0%。结论:各产地射干样品所含黄酮类成分种类相似,成分含量差异较大,应用过程中应加以区分。

Objective：Research on fingerprints of Rhizoma belamcandae of different regions,build mutual pattern mapping,to provide the basis medicinal materials quality control for Rhizoma belamcandae. Methods：Use HPLC method,with acetonitrile and water as mobile phase in gradient elution,detection wavelength of 265 nm. Results：Builded the mutual pattern mapping of 11 samples of Rhizoma belamcandae,the retention time of 25 common chromatographic peaks RSD ≤ 0.77%,and the retention time of other chromatographic peaks RSD〈2.0%. Conclusion：The different origins of Rhizoma belamcandae with similar flavonoids ingredients,but the component content is different,we should differentiate them during the application process.