17β雌二醇对人皮肤成纤维细胞胶原和弹性蛋白合成的影响

Effects of 17β-estradiol on the Synthesis of Collagen and Elastin in Cultured Human Skin Fibroblasts

目的探讨不同浓度17β雌二醇(17β-estrogen,17β-E2)在不同时间点对体外培养的人皮肤成纤维细胞(Human skin fibroblast,h SFB)合成胶原及弹性蛋白的影响。方法体外培养hSFB,分别加入不同浓度的17β-E2(10^(-7)、10^(-8)、10^(-9)、10^(-10)、10^(-11)mol/L),继续培养24 h、48 h、72 h,在不同时间点分别提取相应的RNA,RT-PCR检测经17β-E2处理后的hSFB的Ⅰ、Ⅲ型前胶原及原弹性蛋白mRNA的表达情况。结果 RT-PCR结果显示,17β-E2处理组较空白对照组前胶原及原弹性蛋白表达水平在24 h没有变化,48 h时明显上调,72 h时呈现下降;在48 h时,17β-E2对前胶原与原弹性蛋白的影响,10^(-10) mol/L组上调作用较其他浓度组明显;在48 h时,同一浓度17β-E2对前胶原蛋白的促进作用较原弹性蛋白明显,尤其是对Ⅲ型的促进作用更强。结论 17β-E2对胶原及弹性蛋白的合成有一定的促进作用,不同时间、不同浓度对其影响不同,在10^(-10) mol/l、48 h时作用最强。

Objective To explore the influence of 17 beta estradiol（17β-E2） with different concentrations on the synthesis of collagen and elastin of cultured human skin fibroblasts（h SFB） at different time points in vitro. Methods Human fibroblasts were cultured with different concentrations of 17β-E2（10^（-7）, 10^（-8）, 10^（-9）, 10-10, 10^（-11）mol/L） for 24 hours, 48 hours and 72 hours. The corresponding RNA was extracted at respectively time points, then m RNA expression of type Ⅰprocollagen, type Ⅲ procollagen and tropoelastin were detected by reverse transcription-polymerase chain reaction（RT-PCR）method. Results The m RNA expressions of procollagen Ⅰ, procollagen Ⅲ and tropoelastin were not changed in 24 hours,but up-regulated in 48 hours and declined in 72 hours by 17β-E2 stimulations. On 48 hours, the synthesis of procollagen and tropoelastin stimulated with 10^（-10）mol/L of 17β-E2 was significantly increased, compared with the other concentrations.On 48 hours with the same concentration, the up-regulated effect on synthesis of procollagen and tropoelastin was different,the effect on procollagen was stronger than tropoelastin, especially procollagen Ⅲ. Conclusion 17-E2 has a certain role in promoting the synthesis of collagen and elastin, though the influence is different according to different culture time and different concentration, and the most effective concentration and culture time are 10^（-10）mol/L and 48 hours respectively.