大鼠巨噬细胞感染弓形虫的基因表达谱分析

Gene expression profile analysis of the rat macrophages infected with Toxoplasma gondii

目的分析大鼠腹腔和肺泡巨噬细胞感染弓形虫RH株的基因表达谱差异。方法以弓形虫RH株速殖子感染SD大鼠腹腔和肺泡巨噬细胞0h、6h后提取细胞总RNA,采用NimbleGen 12x135K微阵列基因表达分析芯片检测差异表达基因,并对部分表达变化的基因进行Real time PCR验证。结果表达分析芯片涵盖大鼠26 419个基因,对差异表达基因(Fold Change≥4.0)进行聚类分析显示,经弓形虫RH株作用6h和0h的RNA表达谱相比,大鼠肺泡巨噬细胞上调的基因有49个(主要包括Zfp90,Map4k4,Mrpl42等),下调的基因有130个(主要包括Pitx1,Chpt1,Chd6等)。大鼠腹腔巨噬细胞上调的基因有136个(主要包括Map2k3,Il17b,Phka2等),下调的272个(主要包括Tlr2,Tgfb2,Wnt2等)。GO、Pathway分析差异表达的基因,大鼠腹腔巨噬细胞能够引发更多和弓形虫有关的信号通路变化,其肺泡巨噬细胞则不能引起这一效应。Real time PCR和基因芯片检测结果一致。结论大鼠腹腔和肺泡巨噬细胞感染弓形虫不同的表现型,可能和其基因表达差异引起的信号通路变化有关。

Since rat macrophages are very important for the resistance of Toxoplasma gondii infection,we investigated the differential expression profiling between rat peritoneal and alveolar macrophages by using NimbleGen 12x135 K microarray in this study.Through clustering analysis（Fold Change≥4.0）,the results showed that there were 49 high expression genes（mainly including Zfp90,Map4k4,Mrpl42 and so on）and 130 low expression genes（mainly including Pitx1,Chpt1,Chd6 and so on）in rat alveolar macrophages at the time course of T.Gondii RH strain infection 0hand 6h.As for rat peritoneal macrophages at this condition,there were 136 high expression genes（mainly including Map2k3,Il17 b,Phka2and so on）and272low expression genes（mainly including Tlr2,Tgfb2,Wnt2 and so on）.Further analysis by Go and Pathway methods revealed that rat peritoneal macrophages could trigger more reaction on the related pathway about toxoplamosis,otherwise the alveolar macrophages could not.The results were verified by real-time PCR and the result of verification were entirely coincident.Therefore,the results indicated that the different phenotype between rat alveolar and peritoneal macrophages infected with T.Gondii RH strain may be related to their different expression gene which was connected to toxoplasmosis reaction.