枸杞子乙酸乙酯提取物急性毒性及对切除卵巢大鼠骨质疏松的防治作用

Acute toxicity of ethyl acetate extraction from fructus lycii and its effect on osteoporosis in ovariectomized rats

目的研究枸杞子乙酸乙酯提取物的急性毒性及抗骨质疏松作用。方法枸杞子乙酸乙酯提取物以1.25 g/kg、2.50g/kg、5.00 g/kg、10.00 g/kg对C57/BL6小鼠灌胃给药,连续观察7天,记录急性毒性反应。7天后,观察内脏主要器官的病理变化。将雌性SD大鼠切除卵巢构建绝经后骨质疏松症模型,用125 mg/kg枸杞子乙酸乙酯提取物溶液连续给药3个月,Micro-CT扫描分析检测,评价对去卵巢大鼠骨质疏松症的作用。结果各给药组与对照组小鼠均未见明显的毒性反应症状,枸杞子乙酸乙酯提取物LD50及MTD>10 g/kg。与切除卵巢模型组大鼠相比,枸杞子乙酸乙酯提取物组大鼠的股骨骨密度(P>0.05)、骨体积比(P<0.05)、骨小梁厚度(P>0.05)和骨小梁数目(P<0.05)有所增加,骨小梁间隔(P>0.05)和骨表面积比(P>0.05)有所减小,微观结构各项指标都优于模型组。结论枸杞子乙酸乙酯提取物毒性极小,能够起到一定的抗骨质疏松作用。

Objective To evaluate the acute toxicity and anti-osteoporosis efficacy of ethyl acetate extraction from fructus lycii（ EAEF）. Methods Four doses of EAEF,1. 25,2. 50,5. 00,and 10. 00 g /kg,respectively,were gastricly infused to C57 /BL6 mice for 7 days. The toxic reaction was recorded. After 7 days,the pathological changes of internal organs were observed. The osteoporosis model was induced by ovariectomizing the female SD rats. EAEF of 125 mg / kg was administered for 3 months. The anti-osteoporosis efficacy was analyzed using micro-CT. Results All mice in all groups survived and had no acute symptom of toxicity. The half lethal dose（ LD50） and maximal tolerant dose（ MTD） of EAEF were higher than 10 g /kg. Compared to the osteoporosis model group,bone mineral density（ P〉0. 05）,bone volume fraction（ P〈0. 05）,trabecular thickness（ P〉0. 05）,and trabecular number（ P〈0. 05） increased,but the trabecular spacing（ P〉0. 05） and bone surface area fraction（ P〉0. 05）decreased in the treatment group. The characteristics of microstructure in treatment group were better than those in the osteoporosis model group. Conclusion EAEF has little acute toxicity,and it has the anti-osteoporosis effect.