甲强龙联合脂多糖诱导兔股骨头坏死模型的建立

The model of femoral head necrosis induced by combination of methylprednisolone and lipopolysaccharide in rabbits

目的观察甲强龙联合脂多糖诱导兔股骨头坏死的实验效果。方法 30只健康雄性新西兰兔,随机分为实验组(20只)和对照组(10只),实验组耳缘静脉注射脂多糖(lipopolysaccharides,LPS)10μg/kg,24 h后肌注甲强龙20 mg/kg,1次/d,共3 d。对照组注射同等剂量的生理盐水。6周后观察兔股骨头X射线、磁共振及病理学等指标的改变。结果对照组X线:双侧股骨头骨皮质完整,大小及形态正常,骨密度均匀,骨小梁清晰;实验组X线:兔股骨头密度减低,骨小梁模糊不清,可见一囊状透亮区,股骨头保持完整;MRI示:股骨头见一类圆形高信号影,边界相对清晰,关节面完整;病理:骨小梁纤细,部分骨小梁断裂,髓腔内造血细胞减少,脂肪细胞体积增大,有的融合成泡状。结论甲强龙联合脂多糖能够成功安全便捷地诱导兔股骨头坏死动物模型。

Objective To observe the experimental effect of rabbit femoral head necrosis induced by combination of methylprednisolone and lipopolysaccharide. Methods Thirty male NewZealand rabbits were randomly divided into experimental group（ 20 rabbits） and control group（ 10 rabbits）. Rabbits in the experimental group were injected with 10 μg / kg lipopolysaccharide,and 24 hours later they were injected with methylprednisolone（ 20 mg / kg a day,for 3 days）. Rabbits in the control group were injected with normal saline. X-ray,MRI,and histopathology of the femoral head were examined at 6 weeks after injection. Results The cortical bone of bilateral femoral head was intact,the shape was normal,bone mineral density was even,and the trabecular bone was clear in the control group shown in X-ray image. In experiment group,X-ray showed that the density of the femoral head reduced,the trabecular bone was smear and a cystic translucent zone appeared. The femoral head was kept complete. MRI displayed a round high signal in the femoral head,with relatively clear boundary and complete articular surface.Histopathological： observation showed that the trabecular bone was thin and fractured in some part,the intramedullary hematopoietic cells decreased, and the adipocytes increased in size and fused. Conclusion The osteonecrosis model can be safely and conveniently induced by the combination of dexamethasone and lipopolysaccharide.