洋葱干预后对去势大鼠骨质疏松的影响

The effect of onion intervention on ovariectomy-induced osteoporosis in rats

目的观察洋葱粉对去势大鼠骨密度(bone mineral density,BMD)及血钙、血磷、雌二醇含量变化的影响。方法 3月同龄雌性SD大鼠55只,随机分为对照组(CON)10只,45只进行造模(双侧卵巢切除术)。造模一周后造模组被随机分为模型组(OVX)、低剂量洋葱粉组(LO)、中剂量洋葱粉组(MO)、高剂量洋葱粉组(HO)。分别于12周及24周测量骨密度值,24周后测量血清中钙、磷、雌二醇含量的变化。结果造模12周后,与OVX组比较,MO组、LO组大鼠骨密度值明显增加(P<0.05),HO组大鼠骨密度值略增加,差异无统计学意义(P>0.05);造模24周后,与OVX组比较,MO组、HO组、LO组大鼠骨密度值均明显增加(P<0.05)。MO组、HO组、CON组、OVX组大鼠血清血钙、血磷水平与LO组比较差异具有统计学意义(P<0.05);其它各组两两比较血钙、血磷水平差异无统计学意义(P>0.05)。大鼠血清E2水平由大到小为MO组>HO组>CON组>LO组>OVX组,除LO组大鼠血清E2水平与OVX组和CON组比较差异无统计学意义(P>0.05)外,其它各组两两比较大鼠血清E2水平差异具有统计学意义(P<0.05)。结论洋葱具有提高大鼠雌二醇水平含量,促进雌激素水平分泌,增加BMD作用,达到防治骨质疏松症的目的。

Objective To investigate the effect of onion powder on bone mineral density（ BMD） and serum calcium,phosphorus,and estradiol in ovariectomized rats. Methods Fifty-five female 3-month-old SD rats were divided into two groups,10 rats in control group and 45 rats in ovariectomized group. After 1 week,the ovariectomized group were randomly divided into model group（ OVX）,lowdose onion group（ LO）,middle dose onion group（ MO）,and high dose onion group（ HO）. BMD was measured on 12 weeks and 24 weeks,respectively. Serum levels of calcium,phosphorus,and estradiol were measured on 24 weeks. Results After 12 weeks of ovariectomy,BMD in MO and LO group increased significantly comparing to that in OVX group（ P〈0. 05）. BMD in HO group increased slightly,with no statistical difference（ P〉0. 05）. After 24 weeks,BMD in MO,LO,and HO group elevated significantly comparing to that in OVX group（ P〈0. 05）. The levels of serum calcium and phosphorus in MO,HO,CON and OVX group enhanced significantly,comparing to that in LO group（ P〈0. 05）. The levels of serum calcium and phosphorus had no statistically significant difference between the other groups（ P〉0. 05）. The highest E2 level was in rats of MO group,followed in HO,CON,LO,and OVX group. The difference of E2 levels in rats were statistically significant compared between any two groups,except between that in LO group and OVX and CON group. Conclusion Onion increases E2 level in rats,promotes the secretion of estrogen, elevates the function of BMD, and achieves the goal of preventing and treating osteoporosis.