外源性CGRP诱导糖尿病大鼠膜性成骨的实验研究

Experimental study of induction of periosteum bone formation by exogenous CGRP in diabetic rats

目的探讨外源性降钙素基因相关肽(calcitonin gene related peptide,CGRP)对糖尿病大鼠骨膜微血管病变和膜性成骨的影响。方法建立糖尿病大鼠模型,外源性CGRP静脉注射,随机分为对照组(CON)、糖尿病组(DM)、CGRP干预组(CGRP),分别在5w、10w后观察各组大鼠骨膜微组织结构及组织计量学测定;墨汁灌注观测骨膜微血管单位面积。结果DM1骨祖细胞数较CON均增大(P<0.01),DM2骨膜厚度等均明显小于CON组(P<0.01);微血管单位面积增大,但渗透性大。CGRP骨膜厚度较DM1增多(P<0.01)。CGRP2较DM2骨膜厚度、骨祖细胞数均增大(P<0.01),微血管连续性好。结论外源性CGRP可改善糖尿病大鼠骨膜的微循环损伤,促进膜性成骨对糖尿病大鼠骨质疏松症起到修复作用。

Objective To study the effect of exogenous CGRP on microvascular lesion and intramembranous ossification on periosteum in diabetic rats. Methods The rat diabetic model was established. Exogenous CGRP was intravenously injected. Rats were randomly divided into the control group（ CON）,the diabetic groups（ DM）,and CGRP groups. The microstructure and histomorphometry of the periosteum were observed in 5 weeks and 10 weeks. The microvascular area was determined using ink infusion. Results The number of osteogenitor cells were more in DM1 than those in CON（ P〈0. 01）. The thickness of priosteum was thinner in DM2 than that in CON（ P〈0. 01）. The microvascular area increased but leakage was more. The thickness of periosteum increased in CGRP1 than in DM1（ P〈0. 01）. The number of osteogenitor cells and the thickness of periosteum increased in CGRP2 than those in DM2（ P〈0. 01）,and the microvascular continuity was good. Conclusion The exogenous CGRP relieves the microvascular injury,stimulates intramembranous ossification in periosteum,and repair osteoporosis in diabetic rats.