双醛低聚异麦芽糖交联脱细胞猪真皮基质的研究

Study on procine acellular dermal matrix crosslinked by isomaltooligosaccharide dialdehyde

采用新型交联剂氧化低聚异麦芽糖(IMODA)交联脱细胞猪真皮基质(pADM),优化最佳交联条件,考察了交联材料(IMODA-pADM)的收缩温度、改性指数、傅里叶红外光谱、机械性能、亲水性、吸湿性、表面形貌、耐酶解性能及细胞相容性。结果显示,交联材料的收缩温度随IMODA的用量、反应温度、反应时间的增加而先增大后趋于稳定,随pH值的增加而先增大后减小。交联最优条件为,反应温度37℃、用量16%、反应时间18h、pH值为9.4;随用量增大,交联材料改性指数增大,物理机械性能提高,亲水性与吸湿性略有降低,耐酶解性能大幅提高;交联后材料更为紧实,材料表面有利于细胞粘附生长,细胞形态良好,细胞毒性为0-Ⅰ级。

Porcine acellular dermal matrix （pADM） was crosslinked by a new type crosslinker which is called isomaltooligosaccharide dialdehyde （IMODA）, the crosslinking conditions were optimized, the modification in-dex, fourier transform infrared spectroscopy, mechanical properties, hydrophilicity, scanning electron micros-copy and the cytotoxicity of IMODA-pADM were tested. The results indicated that when the dosage, reactiontemperature and reaction time were increased, the shrinkage temperature was increased firstly and then gradual-ly stayed steady, when the pH value was increased, the shrinkage temperature was increased firstly and then de-creased. It was showed that the comprehensive performance was best when the reaction temperature was 37 ℃,the reaction time was 48 h, dosage was 16% ,pH value was 9.4. After crosslinking, the tensile strength of IMO-DA-pADM was improved, the hydrophilicity was weakened, the surface of the material is conducive to the ad-hesion and growth of cells, and the ctotoxicity grade of crosslinked material was grade 0-I.