不同强度激光对递增负荷运动大鼠心脏炎性损伤和CD8^+T淋巴细胞表达的影响

Effects of laser of different intensities on the myocardial inflammatory injury and CD8^+ T lymphocyte expression of rats exercising with an incremental load

观察递增负荷运动大鼠心脏炎症损伤和CD8^+T淋巴细胞的改变及其关系,探讨不同强度激光治疗对大强度运动心脏的保护作用。8周龄雄性SD大鼠随机分为对照组(C),54只;实验组54只,分为低强度激光+运动组(LE)、高强度激光+运动组(HE)、运动组(E),每组18只。各组分别在2、4、6周末时取材,评定大鼠心脏系数、心脏整体形态、心肌病理积分、心肌CD8^+T淋巴细胞及心肌细胞凋亡情况。结果:与C组比较,E组大鼠6周末时心肌细胞呈现明显炎性浸润,心肌纤维扭曲、肿胀及断裂,病理积分呈显著性差异(P<0.05);大鼠血清TNF-α增加、心肌CD8^+T淋巴细胞浸润、心肌细胞凋亡指数增加呈非常显著性差异(P<0.01)。同时期(2、4、6周末)LE组与E组比较,心肌细胞炎性略轻。6周末时LE组CD8^+T淋巴细胞及心肌细胞凋亡数较C组均上升,而较E组均非常显著性下降(P<0.01)。结果说明低激光治疗可在一定程度上减轻心肌内炎症浸润和致炎性因子,降低心肌病理程度,抑制心肌细胞的凋亡进程。

In oder to observe the changing of and relationship between the myocardial inflammatory injury and CD8~＋ T lymphocyte expression of rats rexercising with an incremental load, and to probe into the function of laser therapy of different intensities on protecting a heat undergoing a high intensity exercise, the authors divided 8-week old male SD rats randomly into a control group（C） consisting of 54 rats, and an experiment group consisting of 54 rats, including a low intensity laser ＋ exercise group（LE）, a high intensity laser ＋ exercise group（HE）, and an exercise group（E）, each of which consisted of 18 rats, sampled the rats in the groups in weeks 2, 4 and 6 respectively, evaluated their heart coefficients, overall heart shapes, myocardial pathological scores, apoptosis of myocardial CD8~＋T lymphocytes and cardiocytes, and revealed the following findings： as compared with the rats in group C, the rats in group E in week 6 showed significant cardiocytes inflammatory infiltrating, myocardial fibers twisting, swelling and rupturing, a significantly different pathological score（P0.01）, increased serum TNF-α, myocardial CD8~＋T lymphocytes filtrating, and a very significantly increased cardiocyte apoptosis index; as compared with the rats in group E in the same period（week 2, 4 or 6）, the rats in group LE had slightly lighter myocardial inflammation; in week 6, the numbers of apoptosis of CD8~＋T lymphocytes and cardiocytes increased as compared with those of the rats in group C, yet decreased significantly as compared with those of the rats in group E（P0.01）. The said find- ings indicate that laser therapy can, to a certain extent, alleviate myocardial inflammatory infiltration and inflammatory factors, reduce the degree of myocardial pathology, and restrain the process of apoptosis of cardiocytes.