人乳头瘤病毒E6/E7mRNA检测宫颈高级别病变的临床价值

宫颈癌是全球发病率居第二位的妇女恶性肿瘤,人乳头瘤病毒（humanpapillomavirus,HPV）的感染与宫颈癌的发病有密切的关系[1].HPV的检测已成为筛查宫颈疾病,预防宫颈癌发生的重要项目.目前实验室常用的HPV检测方法大多是针对高危HPV型的DNA进行检测.HPV感染是宫颈癌的必要条件,但非充分条件.如果没有E6/E7mRNA的转录和表达,即使有高危HPV,仍不会有宫颈癌[2].而HPV-E6/E7mRNA检测能较好地反映HPV致癌基因E6/E7的活动度,可以更为直观地评估宫颈癌的发病风险和预后[3].本文采用支链DNA技术对HPV-E6/E7mRNA进行检测,比较分析其检测宫颈高级别病变的临床价值.

The global incidence of cervical cancer is ranked second in the women＇s cancer, the incidence of HPV （human papilloma virus, HPV） infection and cervicalcancer are closely related [1]. HPV testing has been screened for cervical disease, cervical cancer prevention important projects. HPV detection methodscurrently most commonly used laboratory for testing for high-risk HPV type DNA. Cervical HPV infection is a necessary condition, but not sufficient condition.Without E6 / E7mRNA transcription and expression, even with high-risk HPV, cervical cancer is still there [2]. The HPV-E6 / E7 m RNA detection can reflectthe activity of HPV oncogenes E6 / E7 can be more intuitive assessment of the risk of cervical cancer and prognosis [3]. In this paper, the branched DNAtechnology for HPV-E6 / E7 m RNA detection, a comparative analysis for detecting cervical lesions in the high-level clinical value.