何首乌对血管性认知障碍大鼠学习记忆功能的影响

The Influence of Polygonum on Learning and Memory Function in Rats with Vascular Cognitive Impairment

目的探讨何首乌对血管性认知障碍(VCI)大鼠学习记忆功能的影响。方法筛选造模成功VCI大鼠48只,随机分为4组:何首乌高剂量组、何首乌中剂量组、何首乌低剂量组、VCI模型组,每组12只大鼠。另有12只假手术大鼠作为正常对照组(假手术组)。造模成功后对大鼠用灌胃器灌胃,灌胃液体温度为20℃,何首乌高、中、低剂量组给予4.0 g/m L、2.0 g/m L、0.5 g/m L何首乌溶液灌胃,模型组、假手术组均生理盐水灌胃,灌胃剂量按1 m L/(100 g·d),共28 d。术后28 d各组大鼠开始行Morris水迷宫学习记忆能力等行为学测试,行为学测试结束后,行免疫组织化学检测Bcl-2、Bax阳性细胞的表达。结果与VCI模型组相比,何首乌中剂量组、何首乌高剂量组大鼠逃避潜伏期明显缩短,穿越目标象限时间明显延长,穿越平台次数明显增加,差异有统计学意义(P<0.05或P<0.01);各组VCI大鼠造模28 d完成行为学试验后海马区Bcl-2和Bax蛋白表达显著高于假手术组。而何首乌高剂量组、中剂量组与VCI模型组相比Bcl-2蛋白表达增加的幅度明显大于Bax蛋白(P<0.05或P<0.01)。结论何首乌通过调节Bcl-2、Bax蛋白表达来抑制细胞凋亡,从而改善VCI大鼠的学习以及认知能力。

Objective To investigate the impact of polygonum on learning and memory function in rats with vascular cognitive impairment（ VCI）.Methods Forty-eight VCI model rats were randomly divided into four groups,namely polygonum high dose group,middle dose group,low dose group,VCI model groups.Another 12 sham-operated rats served as normal controls.After a successful modeling by gavage to rats is fed,fed a liquid temperature of 20 ℃,polygonum each treatment group were given different groups according to different concentrations of Polygonum solution mice in the model group,sham group were normal saline irrigation stomach,a total of 28 days.After 28 days the rats began to Morris water maze learning and memory ability behavioral tests,behavioral tests after the end of the line expression of Bcl-2 immunohistochemistry,Bax positive cells.Results Compared with the model group,escape latency was significantly shorter in polygonum middle dose group,high dose group（ P〈0.05 or P〈0.01）.The time of crossing the target quadrant was prolonged,the number of across the platform increased significantly（ P〈0.05 or P〈0.01）.After 28 days of modeling,the expressions of hippocampus Bcl-2 and Bax protein in each group was significantly higher than that in sham group.Compared with the model group,the magnitude of the increase Bcl-2 protein expression was significantly greater than the Bax protein in polygonum middle dose group,high dose group（ P〈0.05 or P〈0.01）.Conclusion Polygonum can inhibit apoptosis by regulating the Bcl-2,Bax protein expression,and improve the learning and cognitive ability in rats with VCI.