TLC法和HPLC法分析黄柏配方颗粒中是否混有关黄柏

TLC and HPLC Analysis of Phellodendri Chinensis Cortex Formula Granules Whether or not Mixed with Phellodendri Amurensis Cortex

目的：用TLC法和HPLC法分析黄柏配方颗粒中是否混有关黄柏。方法：采用薄层色谱法定性鉴别盐酸小檗碱和盐酸巴马汀,高效液相色谱法对盐酸小檗碱进行定量分析。结果：关黄柏与盐酸巴马汀对照品有相应位置的斑点,而黄柏则无。黄柏中所含盐酸小檗碱高于关黄柏3~4倍。结论：方法简便,结果准确,重复性好。

Objective：By TLC and HPLC analysis of Phelldoendri Chinensis Cortex Formula Granules In Whether or not mixed with Phellodendri Amurensis Cortex. Methods：TLC statutory discriminating Berberine Hydrochloride And Palmatine Chloride, HPLC ForBerherine Hydrochloride quantitative analysis. Amurensis Cortex And Palmatine Ch/or/de the reference position corresponding spots, And PheUodendri Chinensis Cortex Noo PheUodendri Chinensis Cor- tex Contained Berbcrine Hydroehloride Higher PheUodendri Amurensis Cortex 3 To 4 Times. Conclusion： This method was found to be simple and accurate.