摘要
为研究芒属观赏草的抗旱性,采用聚乙二醇(PEG)模拟不同程度的干旱胁迫(10%、20%、30%),研究干旱胁迫对1年生芒属4种观赏草生理生化指标的影响。测定其质膜透性,叶绿素a、叶绿素b、可溶性糖、丙二醛(MDA)等的含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(As A-POD)等4种酶活性共9个生理生化指标,并根据测定值判断4种观赏草的抗旱性强弱。结果表明,干旱胁迫下4种芒属观赏草的相对电导率、可溶性糖含量、MDA含量升高;As A-POD活性、叶绿素a、b含量降低;SOD活性、CAT活性、POD活性先升高后降低。对4种观赏草的9个生理生化指标进行方差分析发现,在最高胁迫浓度时,各指标与对照组均存在显著差异(P<0.05)。采用模糊数学隶属函数分析法综合评价,抗旱性强弱顺序为斑叶芒>细叶芒>花叶芒>芒。
To investigate the drought resistance of 4 species of Miscanthus ornamental grass, polyethylene glycol ( PEG) was used to simulate different levels of drought stress ( 10%, 20%, 30%) and make clear the effects of drought stress on the physiological and biochemical indices of 4 species of annual Miscanthus ornamental grass. 9 physiological and biochemical indices such as membrane permeability, chlorophyll a, chlorophyll b, soluble sugar, MDA, SOD, CAT, POD, AsA-POD were measured to assess the drought resistance of 4 species of Miscanthus ornamental grass. With the increase of drought stress, the relative electric conductivities, soluble sugar concentration and MDA increased significantly while AsA-POD, chlorophyll a and chlorophyll b decreased significantly. Activities of SOD, CAT and POD increased and then decreased. According to variance analysis there was significant difference in the indices between the treated groups and the control(P〈0.05) at the highest stress concentration. The drought resistance of 4 ornamental grass species of Miscanthus was evaluated preliminarily with subordinate function analysis and the finally order of the drought resistance was as follows:M. sinensis‘Zebrinus’ 〉M. sinensis‘Gracilimus’ 〉M. sinensis‘Variegatus’〉 M. sinensis Anderss..
出处
《森林与环境学报》
CSCD
北大核心
2016年第2期180-187,共8页
Journal of Forest and Environment
基金
福建省教育厅科技项目(JB13052)
关键词
聚乙二醇6000
干旱胁迫
生理生化指标
综合评价
polyethylene glycol 6000
drought stress
physiological and biochemical indices
comprehensive assessment