^(60)Co-γ辐射对黄花苜蓿出苗和染色体的诱变效应

The Germination and Chromosome Mutagenesis Effect Analysis of ^(60)Co-γ Rays on Medicago falcata L.

用不同剂量(600~1 400Gy,5个梯度,每梯度增加200)的^(60)Co-γ射线辐照吸胀和未吸胀的黄花苜蓿(Medicago falcata L.)种子,通过对出苗、根尖体细胞染色体变异情况的观察,分析辐照对出苗率、叶形态、多倍体诱变率等的影响。结果表明,同剂量辐照下,未吸胀种子的出苗率明显低于吸胀种子出苗率;随辐射剂量增强,第1片真叶长出的时间延长,幼苗生长变缓;^(60)Co-γ射线辐照黄花苜蓿种子其染色体加倍的适宜剂量为1 200Gy。

With different treatments（600,800,1 000,1 200,1 400Gy）of ^（60）Co-γirradiate Medicago falcata L seeds,through observed of the emergence and root tip cells chromosome variation situation,to analysis the irradiation on the germination rate,leaf shape,the influence of polyploid mutation rate,etc.Study showed that after the irradiated,the germination rate of not imbibed seeds is significantly lower than imbibed seed;Along with the enhanced radiation,extend the time of the first true leaf grow,seedling growth slow;1 200 Gy of ^（60）Co-γirradiated alfalfa seed is the appropriate dose.