稻米垩白QTL定位分析

QTL Analysis of Chalkiness in Rice

以高垩白度品种V 20B和低垩白度品种CPSLO 17作为亲本,构建150个V 20B/CPSLO 17重组自交家系(RIL)为作图群体,进行稻米垩白QTL检测及其遗传效应分析。以垩白度为稻米垩白高低的表型数据,结合SLAF标签构建的分子连锁图谱,运用MapQTL 5软件检测到4个稻米垩白QTL,分别命名为qC-5 a、qC-5 b、qC-5 c和qC-5 d。这4个QTL的LOD值分别为4.02、4.09、3.94和4.1,对表型变异的解释率分别为11.6%、11.8%、11.2%和11.8%,且这4个QTL抗性等位基因都来自高垩白亲本V 20B。

A mapping population of 150lines（recombination inbred lines,RIL）,derived from a cross between rice varieties V 20 Band CPSLO 17,was applied to analysis QTLs location of chalkiness trait and evaluated the genetic effects of two parents and 150 RILs.As the chalky degree of RIL populations were used as the phenotype data,the authors constructed a genetic linkage map based on a large number of SLAF markers,and detected 4QTLs（qC-5a、qC-5b、qC-5cand qC-5d）which controll chalkiness degree of rice on chromosome 5by using software Map QTL 5.Individual QTL（LOD=4.02,LOD=4.09,LOD=3.94,LOD=4.1）explained 11.6%、11.8%、11.2% and 11.8% of the observed phenotypic variance separately.All the four of detected QTLs alleles came from high-chalky parent V 20 B.