高效液相色谱波长切换法测定老慢清糖浆中异嗪皮啶、迷迭香酸和柚皮苷的含量

Simultaneous determination of contents of isofraxidin,rosmarinic acid and naringin in Laomanqing syrup by using HPLC wavelength switching method

目的：建立同时测定老慢清糖浆中异嗪皮啶、迷迭香酸和柚皮苷含量的液相色谱测定法。方法：色谱柱为Waters SunFireTMC18（4.6 mm×150 mm,5μm）;以乙腈-0.1%磷酸水溶液（18∶82）为流动相,流速为1.0mL·min-1,柱温：30℃,检测波长：0～12 min为342 nm;12～18 min为284 nm;18～35 min为342 nm。结果：嗪皮啶进样量在0.019～0.335μg范围内线性关系良好（r=0.999 8,n=6）,平均回收率为99.57%,RSD为1.5%（n=9）;迷迭香酸进样量在0.022～0.397μg范围内线性关系良好（r=0.999 9,n=6）,平均回收率为99.55%,RSD为1.3%（n=9）。柚皮苷进样量在0.085～1.531μg范围内线性关系良好（r=0.999 9,n=6）,平均回收率为99.19%,RSD为1.5%（n=9）。结论：该方法准确、快捷、可靠,可更好地用于老慢清糖浆中异嗪皮啶、迷迭香酸和柚皮苷的质量控制。

OBJECTIVE To establish an HPLC wavelength switching method to simultaneously determine contents for isofraxidin,rosmarinic acid and naringin in Laomanqing syrups.METHODS Waters SunFireTMC18（4.6 mm×150 mm,5μm）column was used.Mobile phase was acetonitrile-water containing 0.1% phosphoric acid（18∶82）at a flow rate of 1.0 mL·min-1.Detection wavelength was 342 nm（0-12 min）for isofraxidin,284 nm（12-18 min）for naringin and 342 nm（18-35min）for rosmarinic acid.RESULTS Isofraxidin had a good linearity in the range of 0.019-0.335μg,the average recovery was 99.57% with RSD of 1.5%（n=9）.Rosmarinic acid had a good linearity in the range of 0.022-0.397μg,the average recovery was 99.55% with RSD of 1.3%（n=9）.Naringin had a good linearity in the range of 0.085-1.531μg,and the average recovery was 99.19% with RSD of 1.5%（n=9）.CONCLUSION The method is simple,accurate and reproducible.It can be used to determine contents of isofraxidin,rosmarinic acid and naringin simultaneously in Laomanqing syrups under the same chromatogram conditions.