疸清颗粒对急性肝内胆汁淤积模型大鼠肝功能的保护作用及其机制研究

Study on Protective Effect of Danqing Granules on Liver Function of Rats with Acute Intrahepatic Cholestasis and Its Mechanism

目的:研究疸清颗粒对急性肝内胆汁淤积模型大鼠肝功能的保护作用及其机制。方法:将60只大鼠随机分为空白对照组(生理盐水)、模型1组(生理盐水)、模型2组(生理盐水)、茵栀黄颗粒组(阳性药物,1.62 g/kg)和疸清颗粒高、低剂量组(6.48、1.62 g/kg),每组10只。除正常对照组外,其余各组大鼠均igα-荼基异硫氰酸盐复制急性肝内胆汁淤积大鼠模型,模型1组在造模成功后48 h处死,其余各组大鼠于造模当日开始ig相应药物,每天1次,连续7 d。测定大鼠血清中生化指标[丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TBIL)、直接胆红素(DBIL)、碱性磷酸酶(ALP)及总胆汁酸(TBA)]水平、3 h胆汁流量及流速、血清中脂蛋白(LP-X)浓度及肝组织中Na^+-H^+交换体m RNA的表达。结果:与空白对照组比较,模型1组、模型2组大鼠血清中各生化指标水平均升高、LP-X浓度增加,3 h胆汁流量及流速降低,Na^+-H^+交换体mRNA表达减弱,且模型1组各指标变化幅度大于模型2组(P<0.05);与模型2组比较,各给药组上述指标均得到明显改善(P<0.05)。结论:疸清颗粒对急性肝内胆汁淤积大鼠肝功能具有一定的保护作用,其机制可能与上调肝组织中Na^+-H^+交换体mRNA的表达有关。

OBJECTIVE：To study the protective effect of Danqing granules on liver function of rats with acute intrahepatic cholestasis and its mechanism. METHODS：60 rats were randomly divided into blank control group（normal saline）,model group 1（normal saline）,model group 2（normal saline）,Yinzhihuang granules group（positive drug,1.62 g/kg）,Danqing granules high-dose and low-dose groups（6.48,1.62 g/kg）,with 10 rats in each group. Those group were given α-naphthylisothiocyanate intragastrically to induce acute intrahepatic cholestasis model except normal control group. 48 h after modeling,the rats of model group 1 were sacrificed,and other groups were given relevant medicine intragastrically on the day of modeling,once a day,for consecutive 7 d. The levels of biochemical indexes in serum（ALT,AST,TBIL,DBIL,ALP and TBA）,3 h bile flow and flow velocity,the concentration of LP-X in serum,m RNA expression of Na^＋-H^＋ permutoid were detected in rats. RESULTS：Compared with blank control group,the biochemical indexes and LP-X concentration increased in model group 1 and model group 2,while 3h bile flow and flow velocity and m RNA expression of Na^＋-H^＋permutoid decreased;and the change of each index in model group 1 were more significant than in model group 2（P〈0.05）. Compared with model group 2,above indexes of treatment groups were all improved significantly（P〈0.05）. CONCLUSIONS：Danqing granules have positive effect on liver function of acute intrahepatic cholestasis rats,and its mechanism may be associated with up-regulation of m RNA expression of Na^＋-H^＋ permutoid.