miRNA940调控MDM2/p53通路抑制胶质瘤的增殖

miRNA940inhibits glioma cell proliferation through regulation of MDM2/p53signaling

目的探讨微小核糖核酸940(miRNA940)在胶质瘤组织及细胞株中的表达及其对人胶质瘤细胞增殖的影响。方法采用微阵列芯片技术和荧光定量PCR技术检测miRNA940在胶质瘤组织和细胞中的表达水平。用miRNA940寡聚核苷酸分别转染胶质瘤细胞U87(p53野生型)和U251(p53突变型)细胞系,通过CCK-8增殖检测法和Western blot观察其对胶质瘤细胞增殖能力以及鼠双微基因2(MDM2)、p53和p21蛋白的影响。结果 miRNA940的表达随着胶质瘤恶性程度增加而降低。上调miRNA940后,胶质瘤细胞U87细胞活力降低,MDM2降低,p53和p21升高(P<0.01);但是胶质瘤细胞U251细胞活力和p21未发生明显变化。结论 miRNA940通过调控MDM2/p53从而抑制胶质瘤细胞的增殖能力,提示miRNA940可能参与胶质瘤的发生和发展。

Objective To explore the expression of microRNA940（miRNA940）in glioma tissues and its effect on cell proliferation of glioma cell lines.Methods Microarray technology and FQ-PCR were applied to detect the expression levels of miRNA940 in glioma tissues and glioma cell lines.The miRNA940 oligonucleotides were transfected into glioma cells of U87（p53wild type）and U251（p53 mutanted）.CCK8 proliferation assay was used to evaluate the effect of miRNA940 expression on the proliferation.The changes of mouse double minute 2homolog（MDM2）,p53 and p21proteins were identified with Western blot.Results The expression of miRNA940 was decreased in glioma as the glioma malignancy was elevated.The upregulation of miRNA940 expression could suppress the cell viability,reduce MDM2 protein expression and increase the expressions of p53 and p21.But the cell viability and p21 protein expression of U251 cells did not changed significantly.Conclusion Upregulating miRNA940 expression could inhibit glioma cell proliferation by affecting MDM2/p53,which indicates that miRNA940 may be involved in the occurrence and development of glioma.