HPLC法同时测定夏枯草中熊果酸和齐墩果酸含量

Simultaneous determination of ursolic acid and oleanolic acid in Prunella Vulgaris Linn by HPLC

目的：建立高效液相色谱（HPLC）法同时测定夏枯草中熊果酸和齐墩果酸含量。方法：色谱柱Thermo BDS Hypersil C18（250mm×4．6mm，5μm）；流动相：乙腈-甲醇-0．5％乙酸铵（55：15：30）；流速0．8ml／min；检测波长210nm；柱温：30oC；进样量：20μl。结果：熊果酸进样量在31．81—795．2μg／ml范围内，峰面积与进样量之间线性关系良好（r=0．9998），平均回收率为97．88％；齐墩果酸进样量在33．81—845．2μg/ml范围内，峰面积与进样量之间线性关系良好（r=0．9999），平均回收率为98．39％。结论：本方法可使齐墩果酸、熊果酸得到基线分离，同时测定夏枯草中熊果酸和齐墩果酸的含量。实验结果准确，灵敏度及重复性好，分离度高，为夏枯草的质量控制与评价提供了有效的手段。

Objective ： To establish a quantitative HPLC method for simultaneous determination of ursolic acid and oleanolic acid in Prunella Vulgaris Linn. Method： The separation was performed on a Thermo BDS Hypersil Cls column （250 mm ×4.6 mm, 5 μm）. The mobile phase consisted of acetonitrile -methanol -water- 0.5 % ammonium acetate water solution （55： 15： 30） and the flow rate was 0.8 ml/min. The detection wavelength was at 210 nm. The column temperature was 30℃. Results： The linear ranges of ursolic acid and oleanolic acid were 31.81 - 795.2 μg/ml and 33.81 ~ 845.2 μg/ml, respectively. The average recover- ies of ursolic acid and oleanolic acid were 97.88% and 98.39%, respectively. Conclusion： The method can be used for quality control of Prunella Vulgaris Linn, with a good sensitivity, resolution, accuracy and reproducibility.