茶树根际促生菌的筛选与促生特性的研究

Identification and Characterization of Plant Growth-promoting Rhizobacteria Isolated from Rhizosphere soils of Tea Trees

从贵州地区的茶树根际土壤中分离筛选出6株根际促生菌,经16S rRNA基因分子鉴定,它们分属于假单胞菌属(Pseudomonas sp.)、芽孢杆菌属(Bacillus sp.)、束村氏菌属(Tsukamurella sp.)、伯克霍尔德氏菌属(Burkholderia sp.)和根瘤菌属(Rhizobium sp.)中。促生特性的研究结果显示,溶磷解钾性能最优的菌株为GD-3菌株,固氮效能最高的KKS-6-N1菌株,此外6个菌株具有溶磷解钾固氮、产IAA、产NH3、产HCN能力、可分泌嗜铁素及具有ACC脱氨酶活性等多种促生性能,可作为研制微生物肥料的优良菌株资源。

Based on sequence conservation of 16 S rRNA,six strains of plant growth-promoting rhizobacteria（ PGPR）,which isolated from rhizosphere soils of Guizhou tea trees,were identified as Pseudomonas sp.,Bacillus sp.,Tsukamurella sp.,Burkholderia sp. and Rhizobium sp. GD-3 showed the most efficiency in phosphatesolubilization and potassium decomposition,and KKS-6-N1 had the most efficiency in nitrogen fixing. In addition,other growth-promoting characteristics,e. g. nitrogen fixation,IAA,NH3,HCNproduction,siderophore secretion and 1-aminocyclopropane-1-carboxylic acid（ ACC） deaminase activity,were also investigated in these six strains,justifying their potentials for development ofbiological fertilizer as microbial resources in the future.