辛伐他汀对慢阻肺大鼠MMP-9及其抑制剂表达的影响

Influence of simvastatin on MMP-9 and tissue inhibitor of metalloproteinase-1 in rats with chronic obstructive pulmonary disease

目的研究辛伐他汀对慢性阻塞性肺病大鼠基质金属蛋白酶-9(MMP-9)及其抑制剂(TIMP-1)表达的影响。方法选用30只健康的雄性SD大鼠作为实验材料,将30只大鼠分为三组:A组10只,B组10只,C组10只。B组和C组构建慢阻肺模型,C组大鼠采用辛伐他汀治疗。比较各组大鼠的一般症状、肺功能、支气管肺泡灌洗液(BALF)细胞计数和血清MMP-9、TIMP-1水平。结果 4w后,各组大鼠的体质量、FEV0.3s、FVC、FEV0.3s/FVC、PEV和BALF细胞计数具有统计学差异(P<0.05)。A组大鼠MMP-9为(87.14±21.76)ng/ml,TIMP-1为(82.16±14.89)ng/ml,MMP-9/TIMP-1为(1.07±0.09);B组大鼠MMP-9为(241.58±38.94)ng/ml,TIMP-1为(167.92±19.76)ng/ml,MMP-9/TIMP-1为(1.44±0.21);C组大鼠MMP-9为(134.25±29.15)ng/ml,TIMP-1为(119.41±15.62)ng/ml,MMP-9/TIMP-1为(1.12±0.1),差异具有统计学意义(P<0.05)。结论辛伐他汀可以降低慢阻肺大鼠基质金属蛋白酶-9及其抑制剂的表达水平,并改善两者的比例失衡。

Objective To study the influence of simvastatin on matrix metalloproteinase-9 （ MMP-9 ） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in rats with chronic obstructive pulmonary disease. Methods 30 male SD rats were chosen as study objects, and all rats were divided into 3 groups： 10 in the group A （ control group） , 10 in the group B （ model group） and 10 in the group C （ simvastatin） . After 4 weeks, the symptoms, pul-monary function, cell counting of BALF and level of MMP-9 and TIMP-1 were compared among the 3 groups. Results After 4 weeks, the body mass, FEV0. 3s , FVC, FEV0. 3s/FVC, PEV and cell counting of BALF had statistic differ-ence among the 3 groups （P〈0. 05）. In the group A, MMP-9 was （87. 14 ± 21. 76） ng/ml, TIMP-1was （82. 16 ± 14. 89） ng/ml, and MMP-9/TIMP-1 was （1. 07 ± 0. 09）. In the group B, MMP-9 was （241. 58 ± 38. 94） ng/ml, TIMP-1 was （ 167. 92 ± 19. 76 ） ng/ml, and MMP-9/TIMP-1 was （ 1. 44 ± 0. 21 ） . In the group C, MMP-9 was （134. 25 ± 29. 15） ng/ml, TIMP-1 was （119. 41 ± 15. 62） ng/ml, and MMP-9/TIMP-1 was （1. 12 ± 0. 1）. The difference had statistical significance （P〈0. 05）. Conclusion Simvastatin can reduce the expression level of MMP-9 and TIMP-1 in rats with COPD, as well as regulate their ratio.