摘要
目的探讨脊髓挫伤(SCC)后水通道蛋白-4(AQP-4)在脊髓白质中的表达变化。方法成年健康雌性Sprague-Dawley大鼠88只,随机分为假手术组(Sham)和SCC组。Allen打击法建立SCC模型,BBB评分评价大鼠运动功能变化情况;免疫组织化学染色检测AQP-4在脊髓白质中不同时间点的定位情况;Q-PCR检测AQP-4 m RNA含量变化。结果 SCC组BBB评分明显降低,损伤后7 d和14 d明显升高(t>5.061,P<0.01)。Q-PCR结果显示,术后1 d和3 d AQP-4 m RNA表达显著下降(t>50.44,P<0.001),损伤后5 d,AQP-4 m RNA表达水平较3 d明显上升(t=-3.968,P=0.001),直至28 d仍明显高于3 d(t=-4.227,P=0.001)。免疫组织化学染色结果显示,AQP-4主要定位于脊髓白质的血管内皮细胞和神经胶质细胞的突起。结论 SCC后,AQP-4可能在脊髓损伤后不同时期发挥不同的病理生理作用。
Objective To explore the expression of aquaporin （AQP）-4 in white matter of spinal cord after spinal cord contusion （SCC）. Methods 88 adult Sprague-Dawley rats were assigned randomly to sham operation group and SCC group. The model was established by Al- len＇s method. BBB sore was used to assess the motor function of rats. The relative expression ofAQP-4 mRNA was determined by Q-PCR technique. The localization of AQP-4 was observed by immunohistochemistry. Results BBB score showed motor dysfunction in SCC group, and it increased 7 and 14 days after SCC （t〉5.061, P〈0.001）. The level ofAQP-4 mRNA decreased on the 1st and 3rd days （t〉50.44, P〈 0.001）, and increased on the 5th day （F=-3.968, P=0.001）, and lasted until the 28th day （t=-4.227, P=0.001） compared with that on the 3rd day. The immunohistochemistry showed AQP-4 was located on the process of glial cell and vascular endothelial cells in white matter of spi- nal cord. Conclusion AQP-4 may play various roles at different stages in SCC.
出处
《中国康复理论与实践》
CSCD
北大核心
2016年第4期428-432,共5页
Chinese Journal of Rehabilitation Theory and Practice
基金
四川省级大学生创新创业训练计划项目(No.201313705030)
