SIRT1阻滞剂毒黄素对肺鳞癌细胞株SK-MES-1增殖、凋亡及迁移的影响

Inhibitory effects of toxoflavin on the proliferation, apoptosis and migration of human lung squamous carcinoma cell SK-MES-1

目的研究毒黄素在不同浓度和作用时间对肺鳞癌细胞SK-MES-1增殖、凋亡和迁移的影响。方法取浓度分别为1.0、0.75、0.50、0.25μmol/L毒黄素作用于SK-MES-1肺鳞癌细胞,以不加毒黄素为对照组,培养24和48 h。分别用CCK-8试剂盒、Annexin V-FITC/PI凋亡试剂盒检测细胞生长抑制率和凋亡率。划痕实验对比0.25μmol/L剂量组和对照组在12、24、48 h细胞迁移率。结果在同一毒黄素浓度下,24 h细胞生长抑制率和凋亡率明显小于48 h。在同一时间点,随着毒黄素浓度升高,SK-MES-1肺鳞癌细胞生长抑制率和凋亡率逐渐升高,且都在毒黄素浓度为1.0μmol/L时达到最大值;实验组各时间点SK-MES-1肺鳞癌细胞在12、24、48 h的细胞迁移率明显小于对照组。结论毒黄素对SK-MES-1肺鳞癌细胞有明显生长抑制以及促凋亡作用,并表现出时间和剂量依赖性。毒黄素可抑制SK-MES-1肺鳞癌细胞迁移活性。

Objective To investigate the inhibitory effects of toxoflavin on proliferation, apoptosis and migration of human lung squamous carcinoma cell SK-MES-1. Methods The cultured SK-MES-1 lung squamous carcinoma cells in experimental groups were exposed on toxoflavin（1, 0.75, 0.50 and 0.25 μmol/L） for 24 and 48 h, respectively. The control group was exposed to cell culture media only. CCK-8 assay was used to test the growth inhibitory rate and Annexin V-FITC/propidium iodide（PI） staining assay was used to detect the apoptosis rate of SK-MES-1. The cell migration rate of SK-MES-1 was measured by the wound healing assay at 12, 24, and 48 h. Results The cell growth inhibition rate and apoptosis rate of SK-MES-1 cells at the same concentration of toxoflavin at 48 h were significantly higher than that at 24 h. Furthermore, the cell growth inhibition and apoptosis rate increased along with the increase of toxoflavin concentration at 24 and 48 h, reaching maximal at the concentration of 1.0 μmol/L. Besides, the cell migration rate of SK-MES-1 cells in experimental groups was significantly lower than control group at 12, 24, and 48 h. Conclusion Toxoflavin could significantly inhibit the proliferation and promote apoptosis of SK-MES-1 lung squamous carcinoma cells in a dose- and time-dependent manner, and it could also weaken the migration capacity of the cells.