不同工艺黑木耳多糖的抗氧化与糖苷酶抑制作用研究

Studies on the effects of different polysaccharides extraction process from Auricularia auricula on antioxidant activities and α-glycosidase inhibitory

以黑木耳(Auricularia auricular-judae)子实体为试验对象,为探讨提取工艺对黑木耳多糖对α-葡萄糖苷酶的抑制作用及抗氧化作用的影响,文章分别采用水、纤维素酶以及碱液制备黑木耳多糖,在α-葡萄糖苷酶的最佳反应条件下检测黑木耳多糖对α-葡萄糖苷酶的抑制作用;并对黑木耳多糖的羟基自由基清除率、金属螯合能力及秀丽隐杆线虫的双氧水抗性进行了研究。结果表明,不同提取工艺黑木耳提取物中多糖含量不同,其中碱提黑木耳提取物中糖含量高达50.35%±1.26%,水提黑木耳提取物中糖含量仅为1.65%±0.27%;不同工艺黑木耳提取物对α-葡萄糖苷酶的抑制率及其对体外抗氧化指标和线虫双氧水抗性的影响也有很大差异,其中碱与酶法提取的黑木耳提取物对α-葡萄糖苷酶的抑制率相近(39.24±1.17%与39.61±1.21%),水提取物抑制率仅为13.67±0.97%;酶提取物(2mg/m L)对羟自由基的清除能力及金属螯合力最强,与同浓度水提取物及碱提取物相比差异显著(P<0.05);酶与碱提取物均具有明显增强线虫抗双氧水的能力,在双氧水的氧化刺激下,两种提取物均可以使线虫的存活率延长至与空白对照组一致。

In order to evaluate the effects of different polysaccharides extraction process from the fruiting body of Auricularia auricular-judae on antioxidant activities and α-glycosidase inhibitory,extracts were prepared from Auricularia auricular-judae by water extraction,cellulose enzyme extraction and alkali solution extraction. The polysaccharides content in extracts and the effects of extracts on activity of α-glycosidase,hydroxyl radicals scavenging,chelating effects and Caenorhabditis elegans resistance to H2O2 were determined. The polysaccharides content in extracts from A. auricular-judae with different extraction process are different,and the highest content of 50.35%±1.26% was recorded for alkali solution extraction,and the lowest of 1.65%±0.27% was recorded for water extraction. The inhibition effect on α-glycosidase activity of the extract from the alkali solution extraction was similar to that of the enzyme extraction（39.24±1.17% and 39.61±1.21%）,and the inhibition effect of water extract was only 13.67±0.97%. The highest hydroxyl radicals scavenging and chelating effects were recorded for the extract prepared from the enzyme extraction,and the effects were significant difference compared with the same concentration（2 mg/m L）of water extract and alkali extract（P〈0.05）. In addition,the extracts of enzyme and alkali extraction had significantly enhanced nematodes resistant to H2O2,and they could prolong the nematodes life in the oxidative stress state coincident with control group.