高效液相色谱法测定碳酸饮料中阿力甜含量的不确定度评定

Assessment of the uncertainties in the determination of alitame content in carbonated beverage by UPLC

按照JJF1059.1-2012《测量不确定度评定与表示》的方法,建立高效液相色谱法测定碳酸饮料中阿力甜含量的数学模型,分析其中各不确定度分量的来源,并对其进行分析、量化和合成。通过分析得出,引入不确定度的主要分量有标准溶液的配制操作、标准曲线的拟合过程、试样前处理操作等。碳酸饮料中阿力甜含量测定结果可表示为(0.0979±0.0016)g/kg;k=2。

According to the approach in Measurement Uncertainty and Results Indication（JJF1059.1-2012）,a mathematical model had been set up to assess the uncertainties in the determination of alitame content in carbonated beverage by high performance liquid chromatography（HPLC）. The source of each uncertainty in the mathematical model was analyzed,quantified and synthesized. Results indicated that main influence factors of the uncertainties in the determination were the preparation of standard solution,standard curve fitting process and sample pretreatment. The measurement result of alitame content in carbonated beverage is shown（0.0979±0.0016）g/kg;k=2.