联合检测血清胃蛋白酶原和胸苷激酶1在胃部疾病诊断中的意义

Clinical Significance of Detecting Serum Pepsinogen and Thymidine Kinase 1 Levels in Patients with Gastric Diseases

目的探讨血清胃蛋白酶原（pepsinogen，PG）含量和细胞胸苷激酶1（thymidine kinase1，TK1）水平检测与胃部疾病诊断的关系。方法经胃镜和病理检查诊断浅表性胃炎65例，萎缩性胃炎30例，胃溃疡35例（其中良性胃溃疡15例，恶性胃溃疡20例），胃癌20例，同期健康体检者160例。用乳胶增强免疫比浊法测定血清中的PGI和PGⅡ含量，同时计算出PGI／PGII比值（PGR）。免疫印迹增强化学发光法测定TK1含量。结果对照组和胃部疾病组血清PGI和PGR以及TK1含量差异均有统计学意义（F=88．45～128．71，均P〈0．05）。浅表性胃炎组、萎缩性胃炎组、胃溃疡组和胃癌组之间PGI和PGR以及TK1含量比较差异均有统计学意义（t=16．45～38．71，t=31．25～66．32，t=3．78～8．23，均P〈0．05），而PGII含量比较差异无统计学意义（P〉0．05）。胃溃疡恶性组TK1含量明显比良性组高，且两组比较差异有统计学意义（t=8．45，P〈0．05）。结论联合检测血清PG和TK1含量可诊断胃炎、胃溃疡和胃癌，特别能鉴别胃溃疡良恶性。

Objective To investigate the relationship between serum pepsinogen（PG） levels as well as thymidine kinase 1 （TK1） with Gastric Diseases. Methods 150 cases were selected in the study,superficial gastritis,atrophic gastritis,gastrohelcosis and gastric carcinoma were diagnosed by endoscopy and pathology. And 160 cases were normal subjects. These were measured serum pepsingen Ⅰ, pepsinogen Ⅱ levels by lactoprene immunoturbidimetric assay and calculate PG I/PG Ⅱ ratio （PGR）. TK1 was detected by Chemiluminiseence assay. Results There were obvious differences of PG I , PGR and TK1 levels between gastric diseases group and normal group （F= 88.45 - 128.71, P〈0.05）, however no differences of PG Ⅱ level （P〉0.05）. There were also obviously differences among superficial gastritis group,atrophic gastritis group, gastrohelcosis group and gastric carcinoma group （t =16.45 - 38.71, t = 31.25 - 66.32, t = 3.78 - 8.23, P〈0.05 and t = 8.45, P〈 0. 05）. Conclusion The combined detection of PG level and TK1 are valuable for diagnosis of gastritis,gastrohelcosis and gastric carcinoma and its pathological changes. It is possible for detection of serum TK1 to identify between benign gastrohelcosis and malignancy gastrohelcosis.