SonicFill超声树脂对大鼠成牙本质细胞和牙髓微血管的影响

Effect of SonicFill ultrasonic resin on rat odontoblasts and dental pulp vessels

背景:SonicFill超声树脂在机械性能上优于传统光固化复合树脂充填材料,但其对牙髓成牙本质细胞和牙髓微血管的影响目前尚不清楚。目的:通过组织学方法观察SonicFill超声树脂对大鼠牙髓成牙本质细胞及牙髓微血管的影响。方法:实验选择Wistar大鼠25只,在大鼠的第一和第二磨牙中选取96颗牙齿作为研究对象,随机分为,2组SonicFill超声树脂组和Filtek Z350纳米树脂组,每组48颗实验牙,分为7 d和30 d两个实验周期。另外随机选取5颗非实验牙,不干预,作为正常空白对照。选择上颌第一、二磨牙牙合面制备Ⅰ类窝洞,分别采用SonicFill超声树脂和Filtek Z350纳米树脂进行填充。于充填后7,30 d,墨汁灌注处死大鼠,取牙齿标本,观察牙髓的成牙本质细胞及牙髓微血管的扩张。结果与结论:①充填后7 d:两组均出现相似的牙髓组织改变,表现为牙髓轻度炎症反应,部分标本可见接近洞底牙髓组织中散在的炎症细胞浸润,多数标本可见成牙本质细胞排列紊乱,偶见空泡变性,牙髓微血管有扩张现象。②充填后30 d:SonicFill超声树脂组成牙本质细胞排列基本恢复正常,炎性反应消退,牙髓微血管无扩张,基本恢复正常;Filtek Z350纳米树脂组成牙本质细胞排列紊乱现象减少,炎性反应减轻,牙髓微血管扩张增粗现象减少。③结果表明,两种树脂对成牙本质细胞和牙髓微血管都会产生一定的影响,所产生的影响相似且都较小。

BACKGROUND： The mechanical properties of SonicFill ultrasonic resin are better than those of the traditional light-cured composite resin, but its effect on odontoblasts and dental pulp vessels is unclear. OBJECTIVE： To study the effect of Sonic Fill ultrasonic resin on odontoblasts and dental pulp vessels of rats using histological methods. METHODS： Twenty-five Wistar rats are selected for experiment. Ninety-six teeth from the first and second molars were selected and randomly divided into SonicFill ultrasound resin group and Filtek Z350 nano resin group with 48 teeth in each group. Experimental teeth in each group were equally subdivided into 7 days group and 30 days group. Besides, randomly selected five teeth with no intervention served as normal control group. After anesthesia and disinfection, class I cavities on the occlusal surface of the maxillary first and second molars were prepared and filled with Sonic Fill ultrasound resin and Filtek Z350 nano resin group, respectively. The rats were sacrificed by ink perfusion method, and tooth specimens were taken to observe the changes of odontoblasts and pulp microvessels. RESULTS AND CONCLUSION： At 7 days after filling, two groups had similar changes in the pulp presenting with mild inflammation. Besides, there were scattered inflammatory cells distributing in the cave pulp tissue, and in most of the specimens, odontoblasts arranged disorderly with little vacuoles and microvascular dilatation in the pulp. At 30 days after filling, in the SonicFill ultrasonic resin group, odontoblasts arranged orderly, inflammatory reaction and microvascular dilatation disappeared basically. In contrast, in the Filtek Z350 nano resin group, disordered odontoblasts decreased, inflammatory reaction weakened, as well as microvascular dilatation became less. These results show that under the experimental condition, both of the Sonic Fill ultrasonic resin and Filtek Sonic Fill Z350 nano resin play certain roles in the formation of odontoblasts and dental pulp vessels, but over time all of these effects can automatically disappear.