Tim-3和PD-1在慢性丙型肝炎患者不同单核细胞亚群的表达水平分析

Changes of Tim-3 and PD-1 on peripheral blood monocyte subsets in patients with chronic hepatitis C

目的分析慢性丙型肝炎(CHC)患者外周血单核细胞亚群分布,同时观察负性调节分子T细胞免疫球蛋白及黏蛋白域蛋白3(Tim-3)和程序性细胞死亡蛋白1(PD-1)在各亚群表达的变化及意义。方法利用流式细胞术检测CHC患者外周血单核细胞亚群比例和各亚群Tim-3、PD-1表达,并与临床指标如丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)采用Spearman方法进行相关性分析。结果与健康对照组比较,CHC患者外周血CD14^+CD16^+单核细胞比例增高,以CD14CD16^+单核细胞比例增高显著。Tim-3在CD14CD16-单核细胞和CD14^+CD16单核细胞上表达水平升高;PD-1在CD14CD16-单核细胞和CD14CD16^+单核细胞上表达水平升高。CHC患者单核细胞亚群以及各亚群Tim-3、PD-1和临床指标ALT及AST无明显相关性。结论 Tim-3和PD-1在不同单核细胞亚群表达水平不同。

Objective To investigate the distribution of peripheral blood monocyte subsets of chronic hepatitis C （CHC） patients and observe the expression of negative regulators T cell immunoglobulin and mucin domain-3 （Tim-3） and programmed cell death-1 （PD-1） on the monocyte subsets. Methods Flow cytometry was employed to determine the distribution of three monocyte subsets as well as Tim-3 and PD-1 expression on the three monocyte subsets. Their correlations with the clinical parameters were analyzed by Spearman test. Results Compared with healthy controls, an increased distribution of CD14＋ CD16＋monocytes, especially CDI4＋＋ CD16＋ monocyte subset, was observed in CHC patients. Tim-3 expression was significantly elevated on CD14＋＋CD16＋ and CDI4 ＋ CD16＋＋ subsets in CHC patients. Obviously increased PD-1 expression was found mainly on CD14＋＋ CD16＋and CD14＋＋ CD16＋ subsets. There were no significant correlations between monocyte subsets, PD-1, Tim-3 and the clinical parameters. Conclusion The levels Tim-3 and PD-1 are different in three monocyte subsets.