脑神经胶质瘤患者组织中血管紧张素转移酶高表达的临床意义

Clinical significance of high expression of angiotensin converting enzyme in tumor tissue of patients with brain glioma

目的探讨不同分级的脑神经胶质瘤患者瘤组织中血管紧张素转移酶（ACE）表达及其对预后评估的临床意义。方法采用实时荧光定量聚合酶链反应（PCR）和蛋白印迹（Western blot）法检测48例脑神经胶质瘤组织和22例正常脑组织ACE mRNA和ACE蛋白表达水平。神经胶质瘤分级：Ⅰ级8例;Ⅱ级18例;Ⅲ级16例;Ⅳ级6例。结果脑神经胶质瘤组织中ACE mRNA和蛋白表达水平均明显高于正常脑组织（P〈0.05）。在Ⅲ~Ⅳ级患者的脑神经胶质瘤组织中ACE mRNA表达水平均明显高于Ⅰ~Ⅱ级患者（P〈0.05）。结论 ACE在脑神经胶质瘤患者的瘤组织中呈高表达,与临床病理分级密切相关,提示其对脑神经胶质瘤患者的预后评估有参考价值。

Objective To investigate the expression of angiotensin converting enzyme（ACE）and clinical significance in evaluating the prognosis in patients with different grades of brain glioma.Methods The expression levels of ACE mRNA and protein in 48 patients with different grades of brain glioma and 22 cases of human normal brain tissues were detected by real-time fluorescence quantitative PCR and western blot.According to the criteria of the World Health Organization,all 48 patients with different grades of brain glioma,including 8cases（grade Ⅰ）,18cases（gradeⅡ）,16cases（gradeⅢ）and 6cases（gradeⅣ）,were observed.Results The expression levels of ACE mRNA and protein in brain glioma tissues were significantly higher than those of normal brain tissues（P 0.05）.The expression levels of ACE mRNA in the brain glioma tissues were significantly higher in patients with gradeⅢ-Ⅳthan in patients with gradeⅠ-Ⅱ（P 0.05）.Conclusion ACE is highly expressed in the tumor tissues of patients with brain glioma,which is closely related to tumors grade.These findings suggest that the high expression of ACE in glioma may provide clinical reference value in evaluating the prognosis of the patients with brain glioma.