摘要
以内蒙紫草总黄酮得率为指标,在单因素试验基础上,采用响应面法优化超声波辅助提取工艺,并以芦丁为对照,测定内蒙紫草总黄酮的抗氧化活性。结果表明,响应面试验获得的内蒙紫草总黄酮的最佳提取工艺为乙醇体积分数58.57%,料液比1∶43.33(g/m L),超声时间3.33 h,此时总黄酮得率4.94%。
The objects of this study were to optimize extraction process and to research in vitro antioxidant activity of total flavonoids from Arnebia guttata Bunge. The extraction technology was optimized by single factor experiment and response surface methodology and the antioxidant effect was determined with rutin as positive control. The results had indicated that the extraction optimum technological conditions were the solid-liquid ratio 1 ∶ 43.33（g/m L）,the concentration of ethanol 58.57 %,the extracting time 3.33 h,and the yield of total flavonoids was 4.94 %.
出处
《食品研究与开发》
CAS
北大核心
2016年第5期36-41,共6页
Food Research and Development
基金
广东省自然科学基金项目(S2013010014796)
海珠区科普计划项目(2014HZKP-DS-2)
关键词
内蒙紫草
总黄酮
超声波辅助提取
响应面法
抗氧化活性
Arnebia guttata Bunge
total flavonoids
ultrasonic-assisted extraction
response surface methodology
antioxidant activity
