腐霉利高效降解菌的筛选及其特性

Isolation and characterization of highly efficient procymidone-degrading bacterium

为了降解腐霉利,在长期受农药污染的蔬菜大棚土壤中,驯化分离出13种菌株,通过正交试验确定了混合菌群降解腐霉利的最优条件:腐霉利浓度为300mg/L,葡萄糖浓度为200mg/L,接种量为15%.在分离出的13种菌株中,筛选出1种高效降解腐霉利的菌株T32-1.通过高效液相(HPLC)对菌株T32-1和混合菌群对腐霉利的降解情况进行了分析,结果表明当腐霉利浓度为300mg/L时,菌株T32-1和混合菌群对腐霉利的去除率分别为77.2%和90%.

In order to degradae procymidone,13 species of strains were isolated from the soil in a vegetable greenhouse where was polluted by pesticide for a long time.Based on orthogonal test,the optimal condition of the degradation of procymidone was 300mg/L of initial concentration of procymidone,200mg/L of glucose and 15% of inoculum.Among these strains,strain T32-1shows efficient performance in the degradation of procymidone.The degradation of procymidone by mixed bacterial and strain T32-1were analyzed by HPLC.The results indicated that strain T32-1and mixed bacteria show 77.2% and 90% of high removal efficiency at 300mg/L of initial concentration of procymidone.