中药骨康治疗绝经后骨质疏松症后全基因组miRNA表达谱研究

Whole-genome microRNA expression research in postmenopausal osteoporosis and receiving Gukang treatment

目的研究绝经后骨质疏松症(PMOP)及中药骨康治疗后全基因组微小RNA(miRNA),探讨miRNA表达紊乱与中药治疗PMOP之间的关系。方法提取血浆样本总RNA,应用Illumina高通量测序技术对假手术组、PMOP模型组、骨康方治疗组SD大鼠血浆样本中的miRNA表达水平进行检测,分别比较其miRNA表达的差异。结果 PMOP样本与假手术对照样本之间差异表达的miRNA共829种,其中383种miRNA在PMOP组呈表达上调(17种显著上调),446种呈表达下调(37种显著下调);骨康方治疗组与PMOP对照样本之间差异表达的miRNA共617种,其中192种miRNA在骨康治疗PMOP组呈表达上调(41种显著上调),425种呈表达下调(31种显著下调)。结论多种miRNAs在PMOP以及中药治疗后的血浆样本中异常表达,提示它们可能在PMOP发生发展以及中药治疗中起重要的调控作用。

Objective To investigate the whole-genome micro RNA expression in postmenopausal osteoporosis（PMOP） and receiving Gukang treatment, and the relationship between miRNA expression and PMOP treated by traditional Chinese medicine. Methods Total RNAs were extracted from the plasma samples of PMOP rats, rats with normal BMD（control group） and rats received Gukang treatment, and then were applied to perform global miRNA expression analysis by Illumina deep sequencing technology. Results According to the high-throughput sequencing data, 829 miRNAs were differentially expressed, including 383 up-regulated（17 dramaticlly） and 446 down-regulated miRNAs（37 dramaticlly） in PMOP group compared with control group. 617 differentially expressed miRNAs including 192 up-regulated（41 dramaticlly） and 425 downregulated miRNAs（31 dramaticlly） were indentified in PMOP group after receiving Gukang treatment compared with PMOP group. Conclusion The altered expression profile of miRNA in plasma samples of PMOP rats implies that differentially expressed miRNAs may play roles in the pathogenesis of PMOP.