新疆地区高血压病血液及组织标本库的建立

Establishment of Blood and Tumor Bank of Hypertension in Xinjiang region

目的建立新疆高血压病血液及组织的标本库规范化管理平台,为高血压病在分子水平的研究提供基础。方法选取2006年至2015我院高血压科入院患者,基层流行病学调查入选者,及体检中心健康志愿者,留取血标本,诊断为原发性醛固酮增多症并行肾上腺切除术的患者留取肾上腺腺瘤和瘤旁组织标本,定期提取基因组DNA,使用紫外分光光度计、琼脂糖凝胶电泳、PCR、Taqman基因分型和测序方法检验DNA的质量。使用规范化管理平台对标本进行规范化管理。结果共收集血标本29300人份,DNA标本13300余人份,组织标本151人份,提取基因组DNA,其浓度、A 260/A 280、A 260/A 230以及电泳结果符合质量标准,并能够高效地进行PCR、Taqman基因分型以及测序试验。结论本研究建立了高效的高血压病标本采集流程,能够为今后新疆高血压病的发生发展机制研究提供标本资源。

Objective To establish a standardized specimen bank for blood and tissue of hypertension patients, and to provide a platform for studying hypertension at molecular level. Methods Hospitalized hypertension patients from 2006 to 2015, patients found by epidemiological survey, as well as some healthy volunteers were chosen as study group. Among them, plasma and blood specimens, adrenal adenomas and tumor samples of patients, who had been diagnosed with primary aldosterone and treated with adrenal resection, were collected. DNA extraction kit was used to extract genomic DNA in whole blood and tissues. Agarose gel electrophoresis, PCR test, Taqman genotyping and sequencing experiment were used to test DNA quality. The data information were filed into specimen bank in order to do standardized storage management. Results Whole blood, plasma, serum samples were Collected from 29000 cases, DNA samples were got from 13300 samples, tissue samples were from 151 cases. The blood and tissue samples were periodically extracted genomic DNA, and DNA concentration, A 260 /A 280, A 260/A 230 and agarose gel electrophoresis results were consistent with the quality standard of specimen bank. Genomic DNA can be efficiently determined by PCR, Taq Man gene typing and sequencing tests. Conclusion Hypertension standardized management platform is established through this experiment. And it is proved to be effective and feasibled, the clinical data collection was also complete, which could provide a better specimen resources for studying hypertension better in the future.