骨髓基质干细胞移植阿尔茨海默病模型大鼠:改善学习及记忆能力

Bone marrow mesenchymal stem cells transplanted into a rat model of Alzheimer's disease: improvement in the learning and memory ability

背景:临床上通过药物治疗阿尔茨海默病患者虽然在一定程度上可以减轻和延缓阿尔茨海默病的进展,但单药治疗只有30%的患者达到临床治愈,而且并不能完全康复。目的:观察大鼠骨髓基质干细胞移植对阿尔茨海默病大鼠的治疗作用。方法:将大鼠海马区注射β淀粉样蛋白建立阿尔茨海默病模型大鼠,并在模型大鼠的海马区进行大鼠骨髓基质干细胞移植。结果与结论:(1)行为学变化:造模2周后,与对照组比,模型组和实验组大鼠逃避潜伏期均明显较长(P<0.05),表明阿尔茨海默病模型大鼠建造成功。细胞移植4周后,与模型组比,实验组大鼠平均逃避潜伏期迅速下降(P<0.05),大鼠在平台象限滞留时间明显延长(P<0.05);(2)胆碱乙酰化酶:细胞移植4周后,脑组织检测结果显示,实验组大鼠胆碱乙酰化酶的表达与模型组相比显著提高(P<0.05)。(3)结果显示:骨髓基质干细胞可在阿尔茨海默病大鼠海马区分化及存活,对其学习及记忆能力具有一定的改善作用。

BACKGROUND： Drug therapy can partly reduce and delay the progress of Alzheimer＇s disease, but only 30% with the single drug treatment obtain clinical cure. OBJECTIVE： To study the therapeutic effect of bone marrow mesenchymal stem cells transplantation for rats with Alzheimer＇s disease. METHODS： Amyloid β-protein was injected into the hippocampus of Sprague-Dawley rats to construct the model of Alzheimer＇s disease. And bone marrow stromal stem cells were transplanted into the hippocampus of the rat models. RESULTS AND CONCLUSION： At 2 weeks after modeling, compared with the control group, the escape latency in the model and experimental groups was significantly longer（P 〈0.05）, which indicating that Alzheimer＇s disease models were successfully established. At 4 weeks after cell transplantation, compared with the model group, the average escape latency in the experimental group was significantly decreased, but retention time on the platform quadrant was significantly prolonged（P 〈0.05）. Besides, at 4 weeks after cell transplantation, expression of choline acetyltransferase in the experimental group was significantly higher than that in the model group（P 〈0.05）. In conclusion, bone marrow mesenchymal stem cells cannot only differentiate and survive in the hippocampus of rats with Alzheimer＇s disease, but also improve the learning and memory ability.