CD133+细胞联合人脐血干细胞在心力衰竭小鼠中的作用

CD133^+ cells in combination with human umbilical cord stem cells in mouse heart failure

背景:目前常规方法治疗心力衰竭效果不佳。目的:研究CD133+细胞联合人脐血干细胞在心力衰竭小鼠中的效果,为心力衰竭治疗提供依据。方法:采集足月妊娠顺产婴儿脐血,并利用淋巴细胞分离液法提取CD133+细胞和人脐血干细胞。将20只Balb/C裸小鼠随机等分为单个核细胞组和CD133+细胞联合人脐血干细胞组。2组小鼠均建立心力衰竭模型。单个核细胞组经尾注射单个核细胞治疗,CD133+细胞联合人脐血干细胞组经尾注射CD133+细胞联合人脐血干细胞治疗。结果与结论:(1)治疗后14 d时:CD133+细胞联合人脐血干细胞组小鼠体质量及肝脏、心脏和肺脏质量显著大于单个核细胞组(P<0.05);(2)治疗30 d后:CD133+细胞联合人脐血干细胞组小鼠心肌排列整齐,而单个核细胞组小鼠的心肌排列紊乱,CD133+细胞联合人脐血干细胞组小鼠心肌胶原纤维平均面积显著小于单个核细胞组(P<0.05),血清基质金属蛋白酶9浓度显著低于单个核细胞组(P<0.05),Masson染色结果显示,CD133+细胞联合人脐血干细胞组小鼠蓝色胶原纤维较少,排列相对整齐;单个核细胞组小鼠心内膜出现不同程度胶原纤维增多,排列紊乱;(3)结果提示:CD133+细胞联合人脐血干细胞治疗小鼠心力衰竭效果理想,具有较高的临床应用价值。

BACKGROUND： Currently, conventional treatment methods for heart failure are all ineffective. OBJECTIVE： To explore the combined effects of human umbilical cord stem cells and CD133＋ cells in mice with heart failure, providing a new insight into the treatment of heart failure. METHODS： Full-term newborn umbilical cord from vaginal delivery was collected to isolate CD133＋ cells and human umbilical cord stem cells using lymphocyte separation medium method. Twenty Balb/C nude mice were randomly subjected to mononuclear cell injection（mononuclear cell group） or injection of CD133＋ cells combined with human umbilical cord stem cells（combined group） via the tail vein after establishing heart failure models.RESULTS AND CONCLUSION： Fourteen days after injection, the body weight and liver, heart and lung mass of mice were significantly larger in the combined group than the mononuclear cell group（P〈 0.05）. After 30 days, myocardial cells arranged regularly in the combined group, but disorderly in the mononuclear cell group; compared with the mononuclear cell group, the average area of myocardial collagen fibers was significantly decreased in the combined group（P 〈0.05）, and the level of serum matrix metalloproteinase-9 was also significantly lower in the combined group（P 〈0.05）. Masson staining showed that blue-stained collagen fibers in the combined group were less but arranged neatly; however, in the mononuclear cell group, the number of collagen fibers that arranged irregularly was increased to different extents. To conclude, the combined use of CD133＋ cells and human umbilical cord stem cells has desired outcomes in the treatment of heart failure in mice, indicating a higher clinical value.