基于实时细胞分析技术检测前列腺癌细胞药物敏感性

Assessment of drug sensitivity of the prostate cancer cells based on real-time cell analysis system

目的基于实时细胞分析技术发展,以更加简便稳定的方法进行药物敏感性评估,为前列腺癌临床治疗提供参考。方法选取VCa P、DU145、PC-3、PC-3M-2B4和PC-3M-IE8五株人前列腺癌细胞,选用多西他赛、卡巴他赛和醋酸阿比特龙三种前列腺癌治疗药物,分别梯度浓度给药,利用RTCA检测给药前后的细胞生长情况,确定药物对前列腺癌细胞的半抑制浓度(IC50)。结果多西他赛对VCa P、DU145、PC-3、PC-3M-2B4、PC-3M-IE8五种细胞系24 h的IC50分别为8.81 nmol/L、11.61 nmol/L、1.78 nmol/L、1.44 nmol/L、8.69 nmol/L。卡巴他赛24 h的IC50依次为3.73 nmol/L、3.96 nmol/L、10.41 nmol/L、5.43 nmol/L、7.37 nmol/L。醋酸阿比特龙24 h的IC50依次为8.34μmol/L、8.60μmol/L、24.20μmol/L、8.59μmol/L、13.21μmol/L。结论 PC-3M-2B4及DU145、VCa P及PC-3可作为多西他赛、卡巴他赛及醋酸阿比特龙的对照,建立体外筛选药物的细胞模型,为临床推广提供参考。

Objective To develop a more convenient and stable method for assessment of drug sensitivity of prostate cancer based on real-time cell analysis system as a reference for clinical treatment. Methods Human prostate cancer VCa P,DU145,PC-3,PC-3M-2B4 and PC-3M-IE8 cells were chosen to detect the sensitivity to three drugs,docetaxel,cabazitaxel and abiraterone acetate. Serial dilutions of the three drugs were used to treat the cell culture for 24 hours. The drug-induced effects on the cell lines after an incubation of 24 hours were recorded by the real-time cell analysis system to determine the half maximal inhibitory concentration（ IC50）. Results Docetaxel showd IC50 of 8. 81 nmol / L,11. 61 nmol / L,1. 78 nmol / L,1. 44 nmol / L,8. 69 nmol / L for VCa P,DU145,PC-3,PC-3M-2B4,PC-3M-IE8 cells,respectively. Cabazitaxel showed IC50 of 3. 73 nmol / L,3. 96 nmol / L,10. 41 nmol / L,5. 43 nmol / L,and 7. 37 nmol / L,respectively,for the five cell lines. Abiraterone acetate showed IC50 of 8. 34 μmol / L,8. 60 μmol / L,24. 20 μmol / L,8. 59 μmol / L,and 13. 21 μmol / L for the five cell lines. Conclusions PC-3M-2B4 and DU145,VCa P and PC-3 cells can be used as control for docetaxel,cabazitaxel and abiraterone acetate to establish cell models for the drug screening in vitro and to provide reference for clinical applications.