正常组与人工模拟寒环境组大鼠肺组织蛋白组学差异分析

Normal group and artificial simulation of cold environment proteomics of lung tissue of rats variance analysis

目的:探讨正常组与人工模拟寒环境组大鼠肺组织蛋白组学差异表达及质谱分析。方法:选取20只SD大鼠随机分为正常对照组与寒环境组,每组10只。寒环境组模型复制使用人工气候箱:(T)=(6±2)℃,每天刺激4 h(其余时间为正常组温湿度);正常组(T)=(21±2)℃,每天持续24 h。造模7 d后,两组大鼠于麻醉前12 h禁食、禁水,称量体重。麻醉后开胸取出左肺组织10 mg/只,每组取3个样本进行样品蛋白质制备、双向电泳实验、图像分析、质谱检测。结果:对两组差异蛋白图谱进行蛋白质组学分析,鉴定出9个蛋白在两组之间差异表达,寒环境组与正常对照组比较,其中4个表达上调、5个表达下调。结论:通过蛋白质组学分析,鉴定出的蛋白与外寒环境有相关性。

Objective： To analyse and discuss the variance and mass spectrometry of proteomics of rats＇ lung tissues between groups of normal and simulated cold environments. Methods： A total of 20 SD rats were randomly divided into two groups of normal and simulated cold environments with 10 rats in each group. The temperature of normal environment group was controlled in 21℃ with a fluctuation of 2℃ lasting 24 h per day. In contrast,the temperature of simulated cold environment group was controlled in 6℃ with a fluctuation of 2℃ lasting 4 h per day while the temperature and humidity for the rest of time were exactly the same as the situation in normal environment group. The preoperative fasting,water-deprivation and weighting were adopted 12 h before anaesthesia to both groups after the continuing 7-day experimentation. Left lung tissues of each rat in both groups were removed by the amount of 10 mg,whereas three random samples from each group were tested by experiments of protein extraction,bidirectional electrophoresis,image analysis and mass spectrometry identification. Results： Given the proteomics analysis of two differential sets,it is indicated that in simulated cold environment group,among nine sorts of protein,four sorts perform rising while five falling compared to that of normal group. Conclusion： There exists a correlation between protein and cold environment based on the results of proteomic analysis.