摘要
目的:初步从细胞水平检测原花青素、天麻素、黄芩素、芹菜素四种中药单体对抗原提呈细胞RAW264.7和DC2.4的免疫刺激活性,为肿瘤细胞疫苗佐剂筛选和应用提供理论基础。方法:以LPS作为阳性对照,不同种类、不同浓度的中药单体分别刺激RAW264.7和DC2.4细胞系,48 h后用抗体染色,通过流式细胞仪检测两种细胞表面CD80、CD86、MHCⅠ、MHCⅡ分子的表达量。结果:实验结果显示不同种类、不同浓度的中药单体分别刺激RAW264.7和DC2.4细胞后,细胞表面CD80、CD86、MHCⅠ、MHCⅡ分子不同程度表达上调,原花青素和黄芩素在细胞水平具有较强的免疫刺激活性。结论:原花青素、黄芩素是两种具有潜在应用价值的肿瘤细胞疫苗佐剂。
Objective: To detect immunological effects of procyanidin,gastrodin,baicalein and apigenin on antigen presenting cells of RAW264. 7 and DC2. 4 and search for new tumor vaccine adjuvant. Methods: After different concentrations of four kinds of Chinese herbal monomer and LPS were used to stimulate RAW264. 7 or DC2. 4 cells for 48 hours,cells were obtained and stained with CD80,CD86,MHCⅠ and MHCⅡ antibody. Then protein expression levels of CD80,CD86,MHCⅠ and MHCⅡ on RAW264. 7 and DC2. 4 cells were analyzed by flow cytometry. Results: Results showed that after 48 hours stimulated with different concentrations of four kinds of Chinese herbal monomer on RAW264. 7 or DC2. 4 cells,the expression levels of CD80,CD86,MHCⅠ,MHCⅡ were all up-regulated in comparison with control,procyanidin and baicalein exhibited stronger immunological stimulating activity on a cellular level. Conclusion: Procyanidin and baicalein were found having potential application value in clinical treatment as tumor vaccine adjuvants.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第4期504-508,共5页
Chinese Journal of Immunology
关键词
中药单体
原花青素
黄芩素
免疫活性
筛选
佐剂
Chinese herbal monomers
Procyanidin
Baicalein
Immunological effects
Screening
Adjuvant