柯萨奇病毒A6型VP1蛋白的生物信息学分析

Bioinformatics analysis of VP1 protein of coxsackievirus A6

目的：预测柯萨奇病毒A组6型（Coxsackievirus A6,CVA6）的衣壳蛋白VP1的基本理化性质、结构功能及线性B细胞表位。方法：应用Bioedit软件、Sub Loc、Target P和生物信息学资源门户Ex PASy中的多种在线工具对CVA6 VP1的氨基酸序列进行分析。结果：CVA6 VP1为一亲水性蛋白,其相对分子量为33.6 k D,等电点为7.92,含有24个可能的磷酸化位点,没有信号肽、跨膜区和可能的脂酰化位点;其二级结构中以无规则卷曲居多,有48.52%的氨基酸残基暴露于溶液界面;该分子内存在多个潜在的线性B细胞表位,其中的155～165位氨基酸残基区域的抗原指数最高。结论：成功预测到CVA6 VP1的基本理化性质、结构功能特征及可能的线性B细胞表位,为该蛋白的进一步研究及疫苗和免疫诊断试剂的研制打下基础。

Objective： To predict the basic physicochemical properties,structure and function,and linear B-cell epitopes of the capsid protein VP1 of coxsackievirus A6（ CVA6）. Methods： The amino acid sequence of the CVA6 VP1 was analyzed using Bioedit software and various online tools including Sub Loc,Target P and the others from ExPASy Bioinformatics Resource Portal. Results： The CVA6 VP1 protein was a hydrophilic protein with a relative molecular weight of 33. 6 kD and an isoelectric point of 7. 92. This protein containsed 24 phosphorylation sites,but no signal peptide,transmembrane domains and possible fatty acylation sites. Its secondary structure was characterized by the richest random coils,and 48. 52 percent of its amino acid residues exposed at the solution interface. Epitope prediction by Bepipred showed a number of potential B cell epitopes in the protein,the highest antigenicity index among them located in the region of amino acids residue 155- 165. Conclusion： The basic physicochemical properties,structure and function characteristics,and potential linear B-cell epitopes of CVA6 VP1 were successfully predicted,which laid foundations for the further study on the protein and the preparation of vaccines and immunological diagnostic reagents for CVA6 infection.